Abstract
A simple, scalable method for purification of plasmid DNA is described. Plasmid DNA was released from Escherichia coli JM109 by lysis (1% SDS, 0.2 M NaOH). Then a neutralization solution (3 M sodium acetate buffer, pH 4.8) was added to precipitate genomic DNA and protein. After the clarification of the lysate, the supernatant was placed in a multicompartment electrolyser separated by ultrafilter membranes to remove the remaining contamination (RNA, genomic DNA and protein). A recovery of 75%±2% of total plasmid DNA was obtained after 60 min electrophoresis with a field strength of 8 V cm−1 using cells at 30 g l−1 (quantified by dry cell weight). Genomic DNA, RNA and protein were undetectable in the purified plasmid DNA solution.
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Wang, Z., Le, G., Shi, Y. et al. Purification of plasmid DNA using a multicompartment electrolyser separated by ultrafilter membranes. Biotechnology Letters 24, 121–124 (2002). https://doi.org/10.1023/A:1013839819916
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DOI: https://doi.org/10.1023/A:1013839819916