Abstract
In monocytes/macrophages LPS stimulation occurs by the binding of LPS and the serum component LPS-binding protein (LBP) to CD14. This study was conducted to investigate whether this mechanism also occurs in Kupffer cells. Rat Kupffer cells were stimulated for up to 8 h by LPS (0, 100 ng/ml, 10 μg/ml) in RPMI medium or in nutrient-free Krebs–Henseleit (KH) buffer. Some incubations were performed without serum, while in others serum was provided. TNF-α concentrations of the supernatants were measured by ELISA. LPS stimulation of Kupffer cells yielded the following results. In KH without any additives a considerable amount of TNF-α was released. Incubation in RPMI without serum caused twice as much TNF-α to be released as when KH was used. The addition of autologous serum to RPMI did not increase TNF-α response. These results provide evidence that a substantial part of TNF-α release by LPS-stimulated Kupffer cells occurs in a serum- and thus LBP-independent way.
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Zipfel, A., Schenk, M., Metzdorf, B. et al. Release of TNF-α from Lipopolysaccharide (LPS)-Stimulated Kupffer Cells in Serum- and Nutrient-Free Medium. Inflammation 25, 287–292 (2001). https://doi.org/10.1023/A:1012856408531
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DOI: https://doi.org/10.1023/A:1012856408531