Abstract
Experimental study on the denaturation of enzyme during the separation by foaming was conducted with trypsin and catalase in aqueous medium as model system respectively. The effects of operating pH and sparging gas composition on the denaturation of an enzyme were examined respectively. The oxidative deactivation of enzyme at the gas-liquid interface was identified, which could be reduced by applying nitrogen or carbon dioxide as sparging gas. At suitable conditions, the loss of enzyme activity can be reduced to less than 10% in case of trypsin and to zero in case of catalase. With its proven mildness and effectiveness, foam fractionation in a loop bubble column is applicable for recovery and concentration of enzymes from aqueous solutions.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Beers RF and Sizers Jr. IW (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J. Biol. Chem., 195: 133–140
Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254
Charm ES, Morningstar J, Matteo CC and Paltiel B (1966) The separation and purification of enzymes through foaming. Anal. Chem. 15: 498–508
Farooq Uraizee and Ganesan Narsimhan (1990). Foam fractionation of proteins and enzymes. Enzyme Microb. Technol. 12: 232.
Gehle RD and Schügerl K (1984) Protein recovery by continuous flotation. Appl. Microbiol. Biotechnol. 20: 133–138
Lalchev Z, Dimitrova L, Tzvetkova P and Exerowa D (1982) Foam separation of DNA and proteins. Biotechnol. & Bioeng. 24: 2253–2262
Liu ZH, Liu Z, Shen ZY et al. (1997) Foam separation of proteins in a loop bubble column. Bioseparation 6: 353–359
Montero GA, Kirschner TF and Tanner RD (1993) Bubble and foam concentration of celluse. Appl. Biochem. Biotechnol. 39/40: 467–475
Sarker S, Bhattacharya P, Mukherjea RN and Mukherjea M (1987) Isolation and purification of protease from human placenta by foam fractionation. Biotechnol. Bioeng. 29: 934–940
Schnepf RW and Garden Jr. EL (1959), Foam fractionation of proteins. J. Biochem. Microbiol. Tech. Eng. 1: 1–8
Schwert GW and Takenaka Y (1955) A spectrophotometric determination of trypsin and chymotrypsin. Biochim. Biophys. Acta. 16: 570–575
Uraizee, F. and Narsimhan, G. (1990) Foam fractionation of proteins and enzymes: Enzyme Microb. Technol. 12: 232–233
Zhang LX, Zhang TF and Li LY (1984) Crystallization and determination of trypsin, in Experimental Method and Techniques of Biochemistry (pp 169–175). Chinese Academic Press, Beijing, PR China.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Liu, Z., Liu, Z., Wang, D. et al. On the denaturation of enzymes in the process of foam fractionation. Bioseparation 7, 167–174 (1998). https://doi.org/10.1023/A:1008076818857
Issue Date:
DOI: https://doi.org/10.1023/A:1008076818857