Abstract
Silencing of genes is mostly studied in diploid, homozygous, self-fertile and sexually propagated species. However, conclusions drawn for these species are not always applicable to crops like potato, which is an autotetraploid, highly heterozygous, vegetatively propagated species. Factors influencing the level of silencing in potato are discussed, with emphasis on inhibition of the granule-bound starch synthase I (GBSSI) gene. Type of construct, number of integrated T-DNA copies, structural arrangement of the T-DNA locus, integration site, target tissue and genetic background are important factors for all plant species. Ploidy level and multiple allelism are factors deserving special attention when the efficiency of silencing of endogenous genes is studied in polyploid, heterozygous species such as potato.
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Abel, G.J.W., Springer, F., Willmitzer, L. and Kossmann, J. 1996. Cloning and functional analysis of a cDNA encoding a novel 139 kDa starch synthase from potato (Solanum tuberosum L.). Plant J. 10: 981–991.
Artsaenko, O., Kettig, B., Fiedler, U., Conrad, U. and Düring, K. 1998. Potato tubers as a biofactory for recombinant antibodies. Mol. Breed. 4: 313–319.
Bachem, C.W.B., Speckmann, G.J., van der Linde, P.C.G., Verheggen, F.T.M., Hunt, M.D., Steffens, J.C. and Zabeau, M. 1994. Antisense expression of polyphenol oxidase genes inhibits enzymatic browning in potato tubers. Bio/technology 12: 1101–1105.
Ballicora, M.A., Fu, Y., Nesbitt, N.M. and Preiss, J. 1998. ADPglucose pyrophosphorylase from potato tubers. Site-directed mutagenesis studies of the regulatory sites. Plant Physiol. 118: 265–274.
Dai, W.L., Deng, W., Cui, W.Y., Zhao, S.Y. and Wang, X.M. 1996. Molecular cloning and sequence of potato granule-bound starch synthase gene. Acta Bot. Sinica 38: 777–784.
Davies, H.V. 1996. Recent developments in our knowledge of potato transgenic biology. Potato Res. 39: 411–427.
Davies, H.V. 1998. Prospects for manipulating carbohydrate metabolism in potato tuber. Asp. Appl. Biol. 52: 245–254.
de Carvalho Niebel, F., Frendo, P., Van Montagu, M. and Cornelissen, M. 1995. Post-transcriptional cosuppression of β-1,3–glucanase genes does not affect accumulation of transgene nuclear mRNA. Plant Cell 7: 347–358.
Flipse, E., Huisman, J.G., de Vries, B.J., Bergervoet, J.E.M., Jacobsen, E. and Visser, R.G.F. 1994. Expression of a wild-type GBSS gene introduced into an amylose-free potato mutant by Agrobacterium tumefaciens and the inheritance of the inserts at the microsporic level. Theor. Appl. Genet. 88: 369–375.
Flipse, E., Keetels, C.J.A.M., Jacobsen, E. and Visser, R.G.F. 1996a. The dosage effect of the wildtype GBSS allele is linear for GBSS activity but not for amylose content: absence of amylose has a distinct influence on the physico-chemical properties of starch. Theor. Appl. Genet. 92: 121–127.
Flipse, E., Schippers, M.G.M., Janssen, E.M., Jacobsen, E. and Visser, R.G.F. 1996b. Expression of wild-type GBSS transgenes in the offspring of partially and fully complemented amylose-free transformants of potato. Mol. Breed. 2: 211–218.
Flipse, E., Straatman-Engelen, I., Kuipers, A.G.J., Jacobsen, E. and Visser, R.G.F. 1996c. GBSS T-DNA inserts giving partial complementation of the amylose-free potato mutant can also cause co-suppression of the endogenous GBSS gene in a wild-type background. Plant Mol. Biol. 31: 731–739.
Garcia-Maroto, F., Salamini, F. and Rohde, W. 1993. Molecular cloning and expression patterns of three alleles of the Deficienshomologous gene St-Deficiens from Solanum tuberosum. Plant J. 4: 771–780.
Goddijn, O.J.M. and Pen, J. 1995. Plants as bioreactors. Trends Biotechnol. 13: 379–387.
Heeres, P., Jacobsen, E. and Visser, R.G.F. 1997. Behaviour of genetically modified amylose-free potato clones as progenitors in a breeding program. Euphytica 98: 169–175.
Heyer, A.G., Lloyd, J.R. and Kossmann, J. 1999. Production of modified polymeric carbohydrates. Curr. Opin. Biotechnol. 10: 169–174.
Hofvander, P., Persson, P.T., Tallberg, P.T. and Wikström, O. 1992. Genetically engineered modification of potato from amylopectintype starch. International Patent Application WO 92/11376.
Hovenkamp-Hermelink, J.H.M., Jacobsen, E., Pijnacker, L.P., de Vries, J.N., Witholt, B. and Feenstra, W.J. 1988. Cytological studies on adventitious shoots and minitubers of a monoploid potato clone. Euphytica 39: 213–219.
Hutten, R.C.B. 1994. Basic aspects of potato breeding via the diploid level. Ph.D. thesis, Wageningen Agricultural University, Wageningen, Netherlands.
Jacobs, J.M.E., van Eck, H.J., Arens, P., Verkerk-Bakker, B., te Lintel Hekkert, B., Bastiaanssen, H.J.M., El-Kharbotly, A., Pereira, A., Jacobsen, E. and Stiekema, W.J. 1995. A genetic map of potato (Solanum tuberosum) integrating molecular markers, including transposons, and classical markers. Theor. Appl. Genet. 91: 289–300.
Jobling, S.A., Schwall, G.P., Westcott, R.J., Sidebottom, C.M., Debet, M., Gidley, M.J., Jeffcoat, R. and Safford, R. 1999. A minor form of starch branching enzyme in potato (Solanum tuberosum L.) tubers has a major effect on starch structure: cloning and characterisation of multiple forms of SBE A. Plant J. 18: 163–171.
Khoshnoodi, J., Blennow, A., Ek, B., Rask, L. and Larsson, H. 1996. The multiple forms of starch-branching enzyme I in Solanum tuberosum. Eur. J. Biochem. 242: 148–155.
Krohn, B.M., Hollier, A.A., Darchuk, S. and Stark, D.M. 1998. Improving potato varieties through biotechnology. Asp. Appl. Biol. 52: 239–244.
Kuipers, A.G.J., Vreem, J.T.M., Meyer, H., Jacobsen, E., Feenstra, W.J. and Visser, R.G.F. 1992. Field evaluation of antisense RNA mediated inhibition of GBSS gene expression in potato. Euphytica 59: 83–91.
Kuipers, A.G.J., Jacobsen, E. and Visser, R.G.F. 1994a. Formation and deposition of amylose in the potato tuber starch granule are affected by the reduction of granule-bound starch synthase gene expression. Plant Cell 6: 43–52.
Kuipers, A.G.J., Soppe, W.J.J., Jacobsen, E. and Visser, R.G.F. 1994b. Field evaluation of transgenic potato plants expressing an antisense granule-bound starch synthase gene: increase of the antisense effect during tuber growth. Plant Mol. Biol. 26: 1759–1773.
Kuipers, A.G.J., Soppe, W.J.J., Jacobsen, E. and Visser, R.G.F. 1995. Factors affecting the inhibition by antisense RNA of granule-bound starch synthase gene expression in potato. Mol. Gen. Genet. 246: 745–755.
Kuipers, A.G.J., Jacobsen, E. and Visser, R.G.F. 1997. Applications of antisense technology in plants. In: C. Lichtenstein and W. Nellen (Eds.) Antisense Technology: A Practical Approach, Oxford University Press, Oxford, pp. 191–219.
Kull, B., Salamini, F. and Rohde, W. 1995. Genetic engineering of potato starch composition: inhibition of amylose biosynthesis in tubers from transgenic potato lines by the expression of antisense sequences of the gene for granule-bound starch synthase. J. Genet. Breed. 49: 69–76.
Larsson, C.T., Khoshnoodi, J., Ek, B., Rask, L. and Larsson, H. 1998. Molecular cloning and characterization of starchbranching enzyme II from potato. Plant Mol. Biol. 37: 505–511.
Leonards-Schippers, C., Gieffers, W., Schäfer-Pregl, R., Ritter, E., Knapp, S.J., Salamini, F. and Gebhardt, C. 1994. Quantitative resistance to Phytophthora infestans in potato: a case study for QTL mapping in an allogamous plant species. Genetics 137: 67–77.
Marshall, J., Sidebottom, C., Debet, M., Martin, C., Smith, A.M. and Edwards, A. 1996. Identification of the major starch synthase in the soluble fraction of potato tubers. Plant Cell 8: 1121–1135.
Martinez-Zapater, J.M. and Oliver, J.L. 1984. Genetic analysis of isozyme loci in tetraploid potatoes (Solanum tuberosum L.). Genetics 108: 669–679.
Pilon-Smits, E.A.H., Ebskamp, M.J.M., Jeuken, M.J.W., van der Meer, I.M., Visser, R.G.F., Weisbeek, P.J. and Smeekens, S.C.M. 1996. Microbial fructan production in transgenic potato plants and tubers. Ind. Crops Prod. 5: 35–46.
Rohde, W., Becker, D., Kull, B. and Salamini, F. 1990. Structural and functional analysis of two waxy gene promoters from potato. J. Genet. Breed. 44: 311–315.
Ross, H. 1986. Potato breeding: problems and perspectives. Fortschr. Pflanzenzücht. 13.
Rothacker, D. and Effmert, B. 1968. Zur Problematik der züchterischen Veränderung des Amylose:Amylopektin-Verhältnisses der Kartoffelstärke. Theor. Appl. Genet. 38: 309–313.
Salehuzzaman, S.N.I.M., Jacobsen, E. and Visser, R.G.F. 1993. Isolation and characterization of a cDNA encoding granule-bound starch synthase in cassava (Manihot esculenta Crantz) and its antisense expression in potato. Plant Mol. Biol. 23: 947–962.
Smith, C.J.S., Watson, C.F., Morris, P.C., Bird, C.R., Seymour, G.B., Gray, J.E., Arnold, C., Tucker, G.A., Schuch, W., Harding, S. and Grierson, D. 1990. Inheritance and effect on ripening of antisense polygalacturonase genes in transgenic tomatoes. Plant Mol. Biol. 14: 369–379.
Soltis, D.E. and Soltis, P.S. 1993. Molecular data and the dynamic nature of polyploidy. Crit. Rev. Plant Sci. 12: 243–273.
Sowokinos, J.R., Thomas, C. and Burrell, M.M. 1997. Pyrophosphorylases in potato. V. Allelic polymorphism of UDP-glucose pyrophosphorylase in potato cultivars and its association with tuber resistance to sweetening in the cold. Plant Physiol. 113: 511–517.
van der Leij, F.R., Visser, R.G.F., Osterhaven, K., van der Kop, D.A.M., Jacobsen, E. and Feenstra, W.J. 1991a. Complementation of the amylose-free starch mutant of potato (Solanum tuberosum) by the gene encoding granule-bound starch synthase. Theor. Appl. Genet. 82: 289–295.
van der Leij, F.R., Visser, R.G.F., Ponstein, A.S., Jacobsen, E. and Feenstra, W.J. 1991b. Sequence of the structural gene for granule-bound starch synthase of potato (Solanum tuberosum L.) and evidence for a single point deletion in the amf allele. Mol. Gen. Genet. 228: 240–248.
Visser, R.G.F. 1989. Manipulation of the starch composition of Solanum tuberosum L. using Agrobacterium rhizogenesmediated transformation. Ph.D. thesis, University of Groningen, Netherlands, pp. 97–116.
Visser, R.G.F., Feenstra, W.J. and Jacobsen, E. 1991a. Manipulation of granule-bound starch synthase activity and amylose content in potato by antisense genes. In: J.N.M. Mol and A.R. van der Krol (Eds.) Antisense Nucleic Acids and Proteins: Fundamentals and Applications, Marcel Dekker, New York, pp. 141–155.
Visser, R.G.F., Somhorst, I., Kuipers, G.J., Ruys, N.J., Feenstra, W.J. and Jacobsen, E. 1991b. Inhibition of the expression of the gene for granule-bound starch synthase in potato by antisense constructs. Mol. Gen. Genet. 225: 289–296.
Visser, R.G.F. and Jacobsen, E. 1993. Towards modifying plants for altered starch content and composition. Trends Biotechnol. 11: 63–68.
Visser, R.G.F., Kuipers, A.G.J. and Jacobsen, E. 1996. Antisense granule-bound starch synthase effects in potato. In: D. Grierson, G.W. Lycett and G.A. Tucker (Eds.) Mechanisms and Applications of Gene Silencing, Nottingham University Press, Nottingham, UK, pp. 97–104.
Visser, R.G.F., Suurs, L.C.J.M., Bruinenberg, P.M., Bleeker, I. and Jacobsen, E. 1997a. Comparison between amylose-free and amylose-containing potato starches. Starch/Stärke 49: 438–443.
Visser, R.G.F., Suurs, L.C.J.M., Steeneken, P.A.M. and Jacobsen, E. 1997b. Some physicochemical properties of amylose-free potato starch. Starch/Stärke 49: 443–448.
Wolters, A.M.A., Janssen, E.M., Rozeboom-Schippers, M.G.M., Jacobsen, E. and Visser, R.G.F. 1998a. Composition of endogenous alleles can influence the level of antisense inhibition of granule-bound starch synthase gene expression in tetraploid potato plants. Mol. Breed. 4: 343–358.
Wolters, A.M.A., Trindade, L.M., Jacobsen, E. and Visser, R.G.F. 1998b. Fluorescence in situ hybridization on extended DNA fibres as a tool to analyse complex T-DNA loci in potato. Plant J. 13: 837–847.
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Wolters, AM.A., Visser, R.G. Gene silencing in potato: allelic differences and effect of ploidy. Plant Mol Biol 43, 377–386 (2000). https://doi.org/10.1023/A:1006476621946
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DOI: https://doi.org/10.1023/A:1006476621946