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Plant regeneration from embryogenic cultures of Phragmites australis (Cav.) Trin. Ex Steud.

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Abstract

Embryogenic cultures of the common reed [Phragmites australis (Cav.) Trin. Ex. Steud.] were induced on Murashige and Skoog (1962)-based medium with 2% (w/v) sucrose, B5 vitamins and 4.5 μM 2,4-dichlorphenoxyacetic acid. Four independent culture lines, two initiated from stem nodes and two from roots, were established. These cultures underwent somatic embryogenesis. In one line of stem node origin, the somatic embryos germinated and developed into plants, following transfer of embryogenic cultures to Murashige and Skoog (1962)-based medium lacking growth regulators, with 108 ± 17 plants being recovered per 100 mg fresh weight of culture. In other lines, the somatic embryos developed roots, but not shoots. Shoot regeneration via somatic embryogenesis offers potential as anin vitro system for physiological studies, including assessments of the response of common reed to environmental pollutants.

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Authors and Affiliations

  1. Department of Botany, Kossuth University of Debrecen, 4010-Debrecen, P.O. Box 14, Hungary

    Csaba Máthé, Márta M. Hamvas, István Grigorszky, Gábor Vasas, Erika Molnár & George Borbély

  2. Plant Science Division, School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK

    J. Brian Power, Michael R. Davey & Michael R. Davey

Authors
  1. Csaba Máthé
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  2. Márta M. Hamvas
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  3. István Grigorszky
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  4. Gábor Vasas
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  5. Erika Molnár
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  6. J. Brian Power
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  7. Michael R. Davey
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  8. George Borbély
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Corresponding author

Correspondence to Michael R. Davey.

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Máthé, C., Hamvas, M.M., Grigorszky, I. et al. Plant regeneration from embryogenic cultures of Phragmites australis (Cav.) Trin. Ex Steud.. Plant Cell, Tissue and Organ Culture 63, 81–84 (2000). https://doi.org/10.1023/A:1006471515103

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  • DOI: https://doi.org/10.1023/A:1006471515103

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