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Cell adhesion molecules in subretinal fluid: Soluble forms of VCAM-1 (vascular cell adhesion molecule-1) and L-selectin

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Abstract

Purpose: In this study we investigated the presence of soluble VCAM-1 and soluble L-selectin-1 in subretinal fluids (SRF) of patients suffering from rhegmatogenous retinal detachment . Method: Subretinal fluids were collected from drainage sclerotomies during surgery from 27 patients with rhegmatogenous retinal detachment complicated by proliferative vitreoretinopathy (PVR) or uncomplicated retinal detachment. Levels of sVCAM-1 and sL-selectin-1 were quantified with ELISA. Results: The mean ± SEM values of sVCAM-1 and sL-selectin-1 were 222.2 ± 81 ng/ml and 171.7 ± 42.1 ng/ml, respectively. The concentrations of sVCAM-1 in patients with Grade C PVR (498.2 ± 170 3 ng/ml) were significantly different from those with Grade B PVR (45.6 ± 16.5 ng/ml) and uncomplicated retinal detachments (19.4 ± 12.3 ng/ml). SVCAM-1 concentration in detachments which had been present for more than 8 weeks was 738.8 ± 431 ng/ml, significantly higher than the levels in detachments of shorter duration (132.4 ± 47.7 ng/ml). sL-selectin-1 level in Grade C PVR (291.6 ± 92.8 ng/ml) was higher than in uncomplicated retinal detachments (72.8 ±13.5 ng/ml). Significantly elevated levels of sL-selectin-l were observed in detachments lasting more than 8 weeks (605 ± 151.1 ng/ml) compared to those of shorter duration (96.3 ± 13.1 ng/ml). Conclusion: The present study supports growing evidence that these cell adhesion molecules are involved in the inflammatory process during the development and progression of PVR.

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References

  1. The Silicon Study Group. Proliferative vitreoretinopathy. Am J Ophthalmol 1985; 99: 593¶ 595.

    Google Scholar 

  2. Laqua H, Machemer R. Glial cell proliferation in retinal detachment (massive periretinal proliferation). Am J Ophthalmol 1975; 80: 602¶ 618.

    PubMed  CAS  Google Scholar 

  3. Machemer R, Laqua H. Pigment epithelium proliferation in retinal detachment (massive periretinal proliferation). Am J Ophthalmol 1975; 80: 1¶ 22.

    PubMed  CAS  Google Scholar 

  4. Van Horn D, Aaberg TM, Machemer R, Fenzl R. Glial cell proliferation in human retinal detachment with massive periretinal proliferation. Am J Ophthalmol 1977; 84: 383¶ 393.

    PubMed  CAS  Google Scholar 

  5. Machemer R, Van Horn D, Aaberg TM. Pigment epitelial proliferation in human retinal detachment with massive periretinal proliferation. Am J Ophthalmol 1978; 85: 181¶ 191.

    PubMed  CAS  Google Scholar 

  6. Newsome DA, Rodrigues MM, Machemer R. Human massive periretinal proliferation. In vitro characteristics of cellular components. Am. J. Ophthalmol. 1981; 99: 873¶ 880.

    CAS  Google Scholar 

  7. Hiscott PS, Gierson I, McLeod D. Natural history of fibrocellular epiretinal membranes: an immunohistochemical study. Br J Ophtalmol 1985; 69: 810¶ 823.

    CAS  Google Scholar 

  8. Jerdan JA, Pepose JS, Michels RG, Hayashi H, De Bustros S, Sebag M, Glaser B. Proliferative vitreoretinopathy membranes: An immunohistochemical study. Ophthalmology 1989; 96: 801¶ 810.

    PubMed  CAS  Google Scholar 

  9. Baudin C, Fredj-Reygrobellet D, Gordon W, Baudin F, Peyman G, Lapalus P, Gastaud P, Bazan N. Immunohistologic study of epiretinal membranes in proliferative vitreoretinopathy. Am J Ophthalmol 1990; 110: 593¶ 598.

    Google Scholar 

  10. Vinores SA, Campochiaro PA, Conway P. Ultrastructural and immunohistochemical characterization of of cells in epiretinal membranes. Invest Ophthalmol Vis Sci 1990; 31: 14¶ 28.

    PubMed  CAS  Google Scholar 

  11. Charteris DG, Hiscott P, Grierson I, Lightman SL. Proliferative vitreoretinopathy: Lymphocytes in epiretinal membranes. Ophthalmology 1992; 99: 1364¶ 1367.

    PubMed  CAS  Google Scholar 

  12. Charteris DG, Hiscott P, Robey HL, Gregor ZJ, Lightman SL. Grierson I. Inflammatory cells in proliferative vitreoretinopathy subretinal membranes. Ophthalmology 1993; 100: 43¶ 46.

    PubMed  CAS  Google Scholar 

  13. Baudouin C, Brignole F, Bayle J, Fredj-Reygrobeller D, Lapalus P, Gastaud P. Class II histocompatibility antigen expression by cellular components of vitreus and subretinal fluid in proliferative vitreoretinopathy. Invest Ophthalmol Vis Sci 1991; 32: 2065¶ 2072.

    PubMed  CAS  Google Scholar 

  14. Henley WL, Okas S, Leopold IH. Leucocyte migration inhibition by choroid and retina in retinal detachment. Ophthalmic Res 1975; 7: 129¶ 134.

    Google Scholar 

  15. Brinkman CJ, Broekhuyse RM. Cell-mediated immunity after retinal detachment as determined by lymphocyte stimulation. Am J Ophthalmol 1978; 86: 260¶ 265.

    PubMed  CAS  Google Scholar 

  16. Kaufmann DJ, Van Meurs JC, Mertens DAE, Peperkamp E, Master C, Geritsen ME. Cytokines in vitreus humor: IL-6 is elevated in proliferative vitreoretinopathy. Invest Ophthalmol Vis Sci 1994; 35: 900¶ 906.

    Google Scholar 

  17. Limb GA, Little BC, Meager A, Ogilvie JA, Wolstencroft RA, Franks WA, Chignell AH, Dumonde DC. Cytokines in proliferative vitreoretinopathy. Eye 1991; 5: 686¶ 693.

    PubMed  Google Scholar 

  18. Limb GA, Alam A, Earley O, Green W, Chignell AH, Dumonde DC. Distribution of cytokine proteins in proliferative vitreoretinopathy. Curr Eye Res 1994; 13: 791¶ 798.

    PubMed  CAS  Google Scholar 

  19. Bakunowicz-Lazarczyk A, Moniuszko T, Stankiewicz A. Behaviour of selected cytokines in subretinal fluid. Klin Oczna 1994; 96: 89¶ 90.

    PubMed  CAS  Google Scholar 

  20. Carlos TM, Schwartz BR, Kovach NL, Yee E, Rosa M, Osborn L, Chi-Rosso G, Harlan JM. VCAM-1 mediates lymphocyte adherence to cytokine activated cultured human endothelial cells. Blood 1990; 76: 965¶ 972.

    PubMed  CAS  Google Scholar 

  21. Zimmerman GA, Prescott SM, McIntyre MT. Endothelial cell interactions with granulocytes: tethering and signalling molecules. Immunol Today 1992; 13: 93¶ 99.

    Article  PubMed  CAS  Google Scholar 

  22. The Retina Society Terminology Committee. The classification of retinal detachment with proliferative vitreoretinopathy. Ophthalmology 1983; 90: 121¶ 125.

    Google Scholar 

  23. Feman SS, Lam KW. An enzyme histochemical analysis of human subretinal fluid. Arch Ophthalmol 1978; 96: 129¶ 131.

    PubMed  CAS  Google Scholar 

  24. Dunn SM, Hillam AJ, Stomski F, Jin B, Lucas CM, Boyd AW, Krissansen GW, Burns GF. Leukocyte adhesion molecules involved in inflammation. Transplant Pro 1989; 21: 31¶ 34.

    CAS  Google Scholar 

  25. Carlos TM, Harlan JM. Leukocyte-endothelial adhesion molecules. Blood 1994; 84: 2068¶ 2101.

    PubMed  CAS  Google Scholar 

  26. Tang S, Le-Ruppert KC, Gabel VP. Expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) on proliferating vascular endothelial cells in diabetic epiretinal membranes. Br J Ophthalmol 1994; 78: 370¶ 376.

    PubMed  CAS  Google Scholar 

  27. Platts KE, Benson MT, Rennie IG, Sharrard RM, Rees RC. Cytokine modulation of adhesion molecule expression on human retinal pigment epithelial cells. Invest Ophthalmol 1995; 36: 2262¶ 2269.

    CAS  Google Scholar 

  28. Liversidge J, Sewell HF, Forrester JV. Interactions between lymphocytes and cells of the blood-retina barrier: mechanism of T-lymphocyte adhesion to human retinal capillary endothelial cells and retinal pigment epithelial cells in vitro. Immunology 1990; 71: 390¶ 396.

    PubMed  CAS  Google Scholar 

  29. Duguid IG, Boyd AW, Mandel TE. Adhesion molecules are expressed in the human retina and choroid. Curr Eye Res 1992; 11: 153¶ 159.

    PubMed  Google Scholar 

  30. Heidenkummer HP, Kampik A. Intercellular adhesion molecule-1 (ICAM-1) and leukocyte function associated antigen-1 (LFA-1) expression in human epiretinal membranes. Graefe’s Arch Exp Ophthalmol 1992; 230: 483¶ 487.

    CAS  Google Scholar 

  31. Esser P, Bresgen M, Fischbach R, Heimann K, Wiedemann P. Intercellular adhesion molecule-1 levels in plasma and vitreus from patients with vitreoretinal disorders. German J Ophthalmol 1995; 4: 269¶ 274.

    CAS  Google Scholar 

  32. Gearing AJ, Hemingway I, Pigott R, Hughes J, Rees AJ, Cashman SJ. Soluble forms of vascular adhesion molecules, E-selectin, ICAM-1 and VCAM-1: pathological significance. Ann NY Acad Sd 1992; 667: 324¶ 331.

    CAS  Google Scholar 

  33. Gearing AJ, Newman W. Circulating adhesion molecules in disease. Review Immunol Today 1993; 14: 507¶ 512.

    Google Scholar 

  34. Banks RE, Gearing AJ, Hemingway IK, Norfolk DR, Perren TJ, Selby PJ. Circulating intercellular adhesion molecule-1 (ICAM-1), E-selectin and vascular cell adhesion molecule-1 (VCAM-1) in human malignancies. Br. 3. Cancer 1993; 68: 122¶ 124.

    CAS  Google Scholar 

  35. Cowley HC, Heney D, Gearing AJ, Hemingway I, Webster NR. Increased circulating adhesion molecule concentrations in patients with systemic inflammatory response syndrome. Crit Care Med 1994; 22: 651¶ 657.

    PubMed  CAS  Google Scholar 

  36. Wellicome SM, Kapahi P, Mason JC, Lebranchu Y, Yarwood H, Haskard DO. Detection of a circulating form of vascular cell adhesion molecule-1: raised levels in rheumotoid arthritis and systemic lupus erythematosus. Clin Exp Immunol 1993; 92: 412¶ 418.

    Article  PubMed  CAS  Google Scholar 

  37. Mason JC, Kapahi P, Haskard DO. Detection of increased levels of circulating intercellular adhesion molecule 1 in some patients with rheumotoid arthritis but not in patients with systemic lupus erythematosus. Arthritis Rheum 1993; 36: 519¶ 527.

    PubMed  CAS  Google Scholar 

  38. Billingham MEJ, Cytokines as inflammatory mediators. Brit Med Bull 1987; 43: 350¶ 370.

    PubMed  CAS  Google Scholar 

  39. Tamihara H, Yoshida M, Yoshimura N. Tumor necrosis factor-α gene is expressed in stimulated retinal pigment epithelial cells in culture. Bioch Biophys Res Commun 1992; 187: 1029¶ 1034.

    Article  Google Scholar 

  40. Campochiaro PA. Cytokine production by retinal pigment epithelial cells. Int Rev of Cytol 1993; 146: 75¶ 82.

    CAS  Google Scholar 

  41. Roberge FG, Caspi RR, Nussenblatt RB. Glial retinal Müller cells produce IL-1 activity and have dual effect on autoimmune T helper lymphocytes. J Immunol 1988; 140: 2193¶ 2196.

    PubMed  CAS  Google Scholar 

  42. Bonnet M. Clinical factors predisposing to massive proliferative vitreoretinopathy in rhegmatogenous retinal detachment. Ophthalmologica 1984; 188: 142¶ 152.

    Article  Google Scholar 

  43. Yoshino Y, Ideta H, Nagasaki H, Uemura A. Comparative study of clinical factors predisposing patients to proliferative vitreoretinopathy. Retina 1989; 9: 97¶ 100.

    Article  PubMed  CAS  Google Scholar 

  44. McEver RP. Selectins. Curr Opin Immunol 1994; 6: 75¶ 84.

    Article  PubMed  CAS  Google Scholar 

  45. Kishimoto TK, Jutila MA, Berg EL, Butcher EC. Neutrophil Mac-1 and MEL-14 adhesion proteins inversely regulated by chemotactic factors. Science 1989; 245: 1238¶ 1241.

    PubMed  CAS  Google Scholar 

  46. Smith CW, Kishimoto TK, Abbassi O, Hughes BJ, Rothlein R, McIntyre LW, Butcher EC, Anderson DC. Chemotactic factors regulate lectin adhesion molecule-I (LECAM-1) dependent neutrophil adhesion to cytokine stimulated endothelial cells in vitro. J Clin Invest 1991; 87: 609¶ 618.

    Article  PubMed  CAS  Google Scholar 

  47. Elner VM, Strieter RM, Elner SG, Baggiolini M, Lindley I, Kunkel S. Neutrophil chemotactic factor (IL-8) gene expression by cytokine treated retinal pigment epithelial cells. Am J Pathol 1990; 136: 745¶ 750.

    PubMed  CAS  Google Scholar 

  48. Jaffe GJ, Peters WP, Roberts W, Kurtzberg AS, Wang AM, Stoudemire JB.Modulation of macroghage colony stimulating factor in cultured human epithelial cells. Exp Eye Res 1992; 54: 595¶ 603.

    Article  PubMed  CAS  Google Scholar 

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Authors
  1. Ebru Toker
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  2. Haluk Kazokoĝlu
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  3. Şükran Şahin
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Toker, E., Kazokoĝlu, H. & Şahin, Ş. Cell adhesion molecules in subretinal fluid: Soluble forms of VCAM-1 (vascular cell adhesion molecule-1) and L-selectin. Int Ophthalmol 22, 71–76 (1998). https://doi.org/10.1023/A:1006160209657

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