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Gene delivery for genetically engineered mucosal cells with enhanced function

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Abstract

A non-viral transfection method for oral mucosal cells was investigated using a modified transfection method and five commercial transfection reagents. The CellFECTINTM gave the highest expression of a transfected gene. When the mucosal cells were transfected with 0.3 ng DNA/cell, the transfection efficiency was optimal, and the production of a reporter protein increased up to ten times higher than those with the other transfection reagents.

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References

  • Eming SA, Lee J, Snow RG, Tompkins RG, Yarmush ML, Morgan JR (1995) Genetically modified human epidermis overexpressing PDGF-A directs the formation of a cellular and vascular connective tissue stroma when transplanted to athymic miceimplications for the use of genetically modified keratinocytes to modulate dermal regeneration. J. Invest. Dermtol. 105: 1–8.

    Google Scholar 

  • Eming SA, Snow RG, Yarmush ML, Morgan JR (1996) Targeted expression of insulin-like growth factor to human keratinocyte: modification of the autocrine control of keratinocyte proliferation. J. Invest. Dermtol. 107: 113–120.

    Google Scholar 

  • Garrard AJ, Hudson DL, Brownlee G, Watt FM (1993) Towards gene therapy for hemophilia B by using primary human keratinocytes. Nature Genet. 3: 180–189.

    Google Scholar 

  • Koshizaka T, Hayashi Y, Yagi K (1989) Novel liposomes for effi-cient transfection of _-galactosidase gene into COS-1 cells. J. Clin. Biochem. Nutr. 7: 73–77.

    Google Scholar 

  • Morgan JR, Barradon Y, Green H, Mulligan RC (1987) Expression of an exogenous growth hormone gene by transplantable human epidermal cells. Science 237: 6460–6464.

    Google Scholar 

  • Rheinwald JG, Green H (1975) Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cell. Cell 6: 331–343.

    Google Scholar 

  • Ueda M, Ebata K, Kaneda T (1991) In vitro fabrication of bioarti-ficial mucosa for reconstruction of oral mucosa: basic research and clinical application. Ann. Plastic Surg. 27: 540–549.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan

    Naoki Nagatani, Masashige Shinkai, Yayoi Nagase, Hiroyuki Honda & Takeshi Kobayashi

  2. Department of Oral Surgery, Graduate School of Medicine, Nagoya University, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan

    Ken-ichiro Hata, Hirokazu Mizuno & Minoru Ueda

Authors
  1. Naoki Nagatani
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  2. Masashige Shinkai
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  3. Yayoi Nagase
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  4. Hiroyuki Honda
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  5. Ken-ichiro Hata
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  6. Hirokazu Mizuno
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  7. Minoru Ueda
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  8. Takeshi Kobayashi
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Nagatani, N., Shinkai, M., Nagase, Y. et al. Gene delivery for genetically engineered mucosal cells with enhanced function. Biotechnology Letters 22, 999–1002 (2000). https://doi.org/10.1023/A:1005693321390

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  • DOI: https://doi.org/10.1023/A:1005693321390

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