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Genetic diversity in bambara groundnut (Vigna subterranea (L.) Verdc) landraces assessed by Random Amplified Polymorphic DNA (RAPD) markers

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Abstract

Genetic diversity in 12 landraces of bambara groundnut (Vigna subterranea), an indigenous African legume, was evaluated using Random Amplified Polymorphic DNA (RAPD) markers. DNA from individuals of each landrace was also analysed to determine the level of heterogeneity within landraces. RAPDs revealed high levels of polymorphism among landraces. The percentage polymorphism ranged from 63.2% to 88.2% with an average of 73.1% for the 16 RAPD primers evaluated. The construction of genetic relationships using cluster analysis groups the 12 landraces in two clusters. RAPDs are useful for the genetic diversity studies in V. subterranea and can identify variation within landraces.

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Authors and Affiliations

  1. School of Biosciences, Tropical Crops Research Unit, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK

    F.J. Massawe, J.A. Roberts, S.N. Azam-Ali & M.R. Davey

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  1. F.J. Massawe
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  2. J.A. Roberts
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  3. S.N. Azam-Ali
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  4. M.R. Davey
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Correspondence to F.J. Massawe.

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Massawe, F., Roberts, J., Azam-Ali, S. et al. Genetic diversity in bambara groundnut (Vigna subterranea (L.) Verdc) landraces assessed by Random Amplified Polymorphic DNA (RAPD) markers. Genetic Resources and Crop Evolution 50, 737–741 (2003). https://doi.org/10.1023/A:1025041301787

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