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External accumulation of ions for enhanced electrospray ionization fourier transform ion cyclotron resonance mass spectrometry

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Journal of the American Society for Mass Spectrometry

Abstract

Electrospray ionization (ESI) in combination with Fourier transform ion cyclotron resonance (FTICR) mass spectrometry provides for mass analysis of biological molecules with unrivaled mass accuracy, resolving power and sensitivity. However, ESI FTICR MS performance with on-line separation techniques such as liquid chromatography (LC) and capillary electrophoresis has to date been limited primarily by pulsed gas assisted accumulation and the incompatibility of the associated pump-down time with the frequent ion beam sampling requirement of on-line chromatographic separation. Here we describe numerous analytical advantages that accrue by trapping ions at high pressure in the first rf-only octupole of a dual octupole ion injection system before ion transfer to the ion trap in the center of the magnet for high performance mass analysis at low pressure. The new configuration improves the duty cycle for analysis of continuously generated ions, and is thus ideally suited for on-line chromatographic applications. LC/ESI FTICR MS is demonstrated on a mixture of 500 fmol of each of three peptides. Additional improvements include a fivefold increase in signal-to-noise ratio and resolving power compared to prior methods on our instrument.

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Author notes

  1. Michael W. Senko

    Present address: Finnigan Corp., 355 River Oaks Parkway, 95134, San Jose, CA

Authors and Affiliations

  1. Center for Interdisciplinary Magnetic Resonance, National High Magnetic Field Laboratory, Florida State University, 1800 East Paul Dirac Drive, 32310, Tallahassee, FL

    Michael W. Senko, Christopher L. Hendrickson & Mark R. Emmett

  2. Department of Chemistry, Florida State University, Tallahassee, Florida, USA

    Stone D. H. Shi & Alan G. Marshall

Authors
  1. Michael W. Senko
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  2. Christopher L. Hendrickson
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  4. Stone D. H. Shi
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  5. Alan G. Marshall
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Senko, M.W., Hendrickson, C.L., Emmett, M.R. et al. External accumulation of ions for enhanced electrospray ionization fourier transform ion cyclotron resonance mass spectrometry. J Am Soc Mass Spectrom 8, 970–976 (1997). https://doi.org/10.1016/S1044-0305(97)00126-8

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  • DOI: https://doi.org/10.1016/S1044-0305(97)00126-8

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