Abstract
The trisaccharide unit in lipopolysaccharide of Bordetella pertussis is important for immune recognition, but how the rare sugar 2-acetamido-4-methylamino-fucose in the trisaccharide unit is synthesized and transferred to the core of lipopolysaccharide in B. pertussis remains unclear. In this study, we demonstrated that the genes bplF, bplG, bplL and bplI are involved in the synthesis and transfer of 2-acetamido-4-methylamino-fucose to the core of lipopolysaccharide in B. pertussis. These genes were overexpressed with various combination in a recombinant E. coli strain which can synthesize B. pertussis core-lipid A. Lipopolysaccharides were isolated from these strains and analyzed by using SDS-PAGE, ion chromatography and NMR. Based on these analyses, the mechanism of biosynthesis and transfer of 2-acetamido-4-methylamino-l-fucose to the core-lipid A in B. pertussis is proposed.
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This work is supported by the National Key Research and Development Program of China (2018YFA0900300) and the Postgraduate Research & Practice Innovation Program of Jiangsu Province (CX10295).
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LW and XW: conceived and designed the experiments. LW and ZW: conducted the experiments. LW and XW: analyzed data. XW, XH, ZW and SW: contributed reagents, materials and analysis tools. LW: and XW: wrote the paper. all authors read and approved the manuscript.
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Wang, L., Wang, Z., Wang, S. et al. Biosynthesis and transfer of 2-acetamido-4-methylamino-l-fucose to the core-lipid A in Bordetella pertussis. Syst Microbiol and Biomanuf 3, 273–286 (2023). https://doi.org/10.1007/s43393-022-00106-7
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DOI: https://doi.org/10.1007/s43393-022-00106-7