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Effect of daflon-500®, a flavonoid compound on chlorpyriphos-induced oxidative changes in the hypophysis and testes in adult male rats

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Abstract

Alteration of redox status is one of the molecular pathways commonly associated with pesticide toxicity. Antioxidants, including those obtained from plant phenolics, have been shown to mitigate pesticide-induced cellular injury. The present study was aimed at evaluating the effect of daflon-500®, a flavonoid compound on sub-chronic chlorpyriphos-evoked changes in antioxidant and biochemical parameters in the hypophysis and testes of adult male rats. Twenty-five male albino rats were randomly divided into 5 groups of 5 animals each. Group I (DW) received distilled water (2 ml/kg); group II (SO) was dosed with soya oil (2 ml/kg); Group III (DAF) received daflon-500® at 1000 mg/kg ̴ 1/5th of LD50 (≥ 5000 mg/kg); group IV (CP) was administered chlorpyriphos at 7.74 mg/kg ̴ 1/10th of LD50 (77.4 mg/kg) while group V (DAF + CP) was previously treated with daflon-500® (1000 mg/kg) and then exposed to CP (7.74 mg/kg), 30 min later. Daily oral regimen administration was done for 60 days after which the animals were sacrificed by cervical venesection after light chloroform anesthesia. The hypophysis and testicular tissues were harvested, and their homogenates were analyzed for malondialdehyde, catalase and superoxide dismutase, and acetylcholinesterase levels. A significant increase in the hypophysis and testicular MDA concentrations, coupled with a decrease in the SOD, CAT, and AChE activities were observed in the CP group. The levels of these oxidative and biochemical parameters were alleviated in the group pretreated with Daflon-500®. Results of this study demonstrated that pre-treatment with Daflon-500® mitigated CP-induced alterations in oxidative and biochemical parameters apparently due to the antioxidant effect of the flavonoid compound.

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Abbreviations

ACh:

Acetylcholine

AChE:

Acetylcholinesterase

CAT:

Catalase

CP:

Chlorpyriphos

DAF:

Daflon-500®

DW:

Distilled water

H2O2 :

Hydrogen peroxide

MDA:

Malondialdehyde

OPs:

Organophosphates

ROS:

Reactive oxygen species

SO:

Soya oil

SOD:

Superoxide dismutase

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Acknowledgements

We acknowledged the staff of the Department of Veterinary Pharmacology and Toxicology, Ahmadu Bello University, Zaria especially Mr. D. Otie for his laboratory expertise during the period of this study.

Funding

No funding was received for conducting this study.

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Authors and Affiliations

  1. Veterinary Pharmacology and Toxicology, University of Ilorin, Ilorin, Kwara, Nigeria

    Aishat O. Olatunji, Suleiman F. Ambali, Ganiu J. Akorede & Khalid T. Biobaku

  2. Veterinary Physiology, Ahmadu Bello University, Zaria, Kaduna, Nigeria

    Joseph O. Ayo

  3. Veterinary Pharmacology and Toxicology, Ahmadu Bello University, Zaria, Kaduna, Nigeria

    Mohammed M. Suleiman & Muftau Shittu

  4. Theriogenology and Production, University of Ilorin, Ilorin, Kwara, Nigeria

    Lukman O. Raji

  5. Veterinary Pathology, University of Ilorin, Ilorin, Kwara, Nigeria

    Jamila A. Atata

  6. Veterinary Physiology and Biochemistry, University of Ilorin, Ilorin, Kwara, Nigeria

    Mistura O. Azeez

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  1. Aishat O. Olatunji
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Correspondence to Aishat O. Olatunji.

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Olatunji, A.O., Ayo, J.O., Suleiman, M.M. et al. Effect of daflon-500®, a flavonoid compound on chlorpyriphos-induced oxidative changes in the hypophysis and testes in adult male rats. Toxicol Res. 38, 345–353 (2022). https://doi.org/10.1007/s43188-021-00120-2

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  • DOI: https://doi.org/10.1007/s43188-021-00120-2

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