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Characterization of two constitutive promoters RPS28 and EIF1 for studying soybean growth, development, and symbiotic nodule development

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Abstract

Native promoters that can drive high and stable transgene expression are important tools for modifying plant traits. Although several such promoters have been reported in soybean (Glycine max), few of them function at multiple growth and development stages and during nodule development. Here, we report that the promoters of 40S RIBOSOMAL PROTEIN SMALL SUBUNIT S28 (RPS28) and EUKARYOTIC TRANSLATION INITIATION FACTOR 1 (EIF1) are ideal for high expression of transgene. Through bioinformatic analysis, we determined that RPS28 and EIF1 were highly expressed during soybean growth and development, nodule development, and various biotic and abiotic stresses. Fusion of both RPS28 and EIF1 promoters, with or without their first intron, with the reporter gene β-GLUCURONIDASE (uidA) in transgenic soybean, resulted in high GUS activity in seedlings, seeds, and nodules. Fluorimetric GUS assays showed that the RPS28 promoter and the EIF1 promoter yielded high expression, comparable to the soybean Ubiquitin (GmUbi) promoter. RPS28 and EIF1 promoters were also highly expressed in Arabidopsis thaliana and Nicotiana benthamiana. Our results indicate the potential of RPS28 and EIF1 promoters to facilitate future genetic engineering and breeding to improve the quality and yield of soybean, as well as in a wide variety of other plant species.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This work was supported by National Natural Science Foundation of China (Grant No. 31870257 and U21A20181 to X. W., Grant no. 32170728 to H. W.), the National Key Research and Development Program (Grant No. 2018YFE0112100 to X. W.), Excellent Youth Foundation of Henan Province (Grant No. 222300420025 to H. W.), and the 111 Project of China (Grant No. D16014).

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Author notes

  1. Shengcai Chen and Yaqi Peng have contributed equally to this work.

Authors and Affiliations

  1. College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Shengcai Chen & Jing Liu

  2. State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Key Laboratory of Plant Stress Biology, Henan University, Kaifeng, 475001, China

    Yaqi Peng, Qi Lv, Jing Liu, Haijiao Wang & Xuelu Wang

  3. Sanya Institute of Henan University, Sanya, 572025, China

    Yaqi Peng, Qi Lv, Jing Liu, Haijiao Wang & Xuelu Wang

  4. Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China, Key Laboratory of State Ethnic Affairs Commission for Biological Technology, College of Life Sciences, South-Central University for Nationalities, Wuhan, 430074, China

    Zhihua Wu

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  1. Shengcai Chen
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  2. Yaqi Peng
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  3. Qi Lv
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  4. Jing Liu
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Correspondence to Haijiao Wang or Xuelu Wang.

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The authors have no conflict of interest declared. Author Xuelu Wang was not involved in the journal’s review of this manuscript.

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Chen, S., Peng, Y., Lv, Q. et al. Characterization of two constitutive promoters RPS28 and EIF1 for studying soybean growth, development, and symbiotic nodule development. aBIOTECH 3, 99–109 (2022). https://doi.org/10.1007/s42994-022-00073-6

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