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Distribution of the non-isoprene components in the four Hevea brasiliensis latex centrifugation fractions

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Abstract

This work is part of a larger project aiming at elucidating the mechanisms by which the biochemical components naturally occurring in Hevea brasiliensis latex influence the structuration of natural rubber (NR). To achieve this overall objective, our strategy consists of measuring the physical properties of NR samples made of reconstructed lattices which were obtained by mixing different proportions of C-serum and bottom fraction (lutoids) to rubber fraction made of small and large particles. This work represents the first step of the above-mentioned project where we carried out a systematic study to identify and locate the main biochemical components of latex that might drive NR structuration. Fresh latex from RRIM600 and PB235 clones was fractionated by centrifugation into 4 fractions: large rubber particles, small rubber particles, C-serum and bottom fraction (lutoids + Frey-Wyssling particles). The fractions were further analyzed to provide a comprehensive description of their biochemical composition (lipids, proteins, minerals) in comparison with the original whole latex. The results gathered in this work are essential for further study of the quantitative influence of each component on NR structuration and properties.

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Acknowledgements

The authors thank ANR agency for financial support of the RUBBex-ANR 14-CE07-0026-02 project. Thailand International Cooperation Agency (TICA) is acknowledged for financial support of the field trips. The authors would like to thank Visahakit Thai Rubber Co., Ltd. for kindly providing samples. Agropolis Fondation is thanked for the support to the creation of LipPolGreen-Asia platform in Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Bangkok. This work was undertaken as part of the Hevea Research Platform in Partnership (HRPP).

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Authors and Affiliations

  1. KAPI, Kasetsart University, Bangkok, 10900, Thailand

    Siriluck Liengprayoon, Saowalak Jantarasunthorn, Jatuporn Chaiyut & Natedao Musigamart

  2. CIRAD, UPR BioWooEB, 34398, Montpellier, France

    Laurent Vaysse, Christine Char, Frederic Bonfils & Céline Bottier

  3. BioWooEB, Univ Montpellier, CIRAD, Montpellier, France

    Laurent Vaysse, Christine Char, Frederic Bonfils & Céline Bottier

  4. IFRPD, Kasetsart University, Bangkok, 10900, Thailand

    Kanthida Wadeesirisak

  5. Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok, 10900, Thailand

    Jatuporn Chaiyut, Surattiya Srisomboon & Kittipong Rattanaporn

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  1. Siriluck Liengprayoon
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Correspondence to Siriluck Liengprayoon or Céline Bottier.

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Liengprayoon, S., Vaysse, L., Jantarasunthorn, S. et al. Distribution of the non-isoprene components in the four Hevea brasiliensis latex centrifugation fractions. J Rubber Res 24, 759–769 (2021). https://doi.org/10.1007/s42464-021-00133-4

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