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Cardio-Protective Effects of Germinated Brown Rice Extract Against Myocardial Ischemia Reperfusion Injury

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Abstract

Introduction

Ischemic heart disease is closely associated with many risk factors. Germinated brown rice extract (GBR) has potent antioxidant activities for alleviating the factors for developing heart failure such as hypertension and diabetes mellitus.

Aim

The objective of the present study was to determine the cardio-protective effects of GBR and to elucidate the mechanisms underlying these effects in a model of simulated myocardial ischemic/ reperfusion injury (sI/R).

Methods

An in vitro study was performed on cultured rat cardiomyoblasts (H9c2) exposed to sI/R. The expression of apoptosis and signaling proteins was assessed using Western blot analyses. Eighteen New Zealand White rabbits were divided into 3 groups and the left circumflex coronary artery was ligated to induce myocardial ischemia. Heart functions were monitored by electrocardiography and echocardiography 0, 30, and 60 days after coronary artery ligation.

Results

GBR consumption group showed significantly improved cardiac function and reduced the heart rate, along with reduced mean arterial pressure and plasma glucose level. Also, GBR showed good scavenging activity, pretreatment with GBR inhibited I/R induced apoptosis by suppressing the production of caspase 3 and p38 MAPK.

Conclusions

These results suggest that intake of germinated brown rice may effectively to protect cell proliferation and apoptosis and may provide important nutrients to prevent heart failure due to myocardial ischemia.

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Authors and Affiliations

  1. Department of Large Animal and Wildlife Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen Campus, Bangkok, 73140, Thailand

    Soontaree Petchdee

  2. Department of Chemistry, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Bangkok, 73140, Thailand

    Wanpen Laosripaiboon & Nongpanga Jarussophon

  3. Department of Medical Technology, Faculty of Allied Health Science, Naresuan University, Phitsanulok, 65000, Thailand

    Sarawut Kumphune

Authors
  1. Soontaree Petchdee
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  2. Wanpen Laosripaiboon
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  3. Nongpanga Jarussophon
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  4. Sarawut Kumphune
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Corresponding author

Correspondence to Soontaree Petchdee.

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The authors declare no conflict of interest.

Funding statement

This work was supported by National Research Council of Thailand (NRCT) and Agricultural Research Development Agency (ARDA) [Grant number PRP5705021410, 2014].

Ethical approval

The study was conducted in New Zealand white rabbits, and approved by the Ethical Committee for Animal Experiments, Kasetsart University, Thailand (ACKU 04059).

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Petchdee, S., Laosripaiboon, W., Jarussophon, N. et al. Cardio-Protective Effects of Germinated Brown Rice Extract Against Myocardial Ischemia Reperfusion Injury. High Blood Press Cardiovasc Prev 27, 251–258 (2020). https://doi.org/10.1007/s40292-020-00378-x

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  • DOI: https://doi.org/10.1007/s40292-020-00378-x

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