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Development of a Two-photon Ratiometric Fluorescent Probe for Glutathione and Its Applications in Living Cells

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Abstract

Glutathione(GSH), as the most abundant intracellular biothiol, plays an important role in the redox homeostasis of the organism. Abnormal concentrations of GSH in cells may lead to many malignant diseases, such as cancer, liver damage and neurodegenerative diseases. It is urgent to develop effective methods to detect GSH in living organisms. In this work, a new two-photon ratiometric fluorescent probe Co-GSH based on the coumarinchalcone dye platform was judiciously developed. Based on the Michael-addition reaction, Co-GSH was able to identify GSH with high selectivity and sensitivity. Furthermore, assisted by laser-scanning confocal microscopy, Co-GSH could specifically response GSH over the other biothiols, including Cys and Hcy, in living HeLa cells by using one- and two-photon modes.

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Authors and Affiliations

  1. Institute of Fluorescent Probes for Biological Imaging, School of Materials Science and Engineering, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, P. R. China

    Gaoping Xu, Yonghe Tang & Weiying Lin

Authors
  1. Gaoping Xu
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  2. Yonghe Tang
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  3. Weiying Lin
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Corresponding author

Correspondence to Weiying Lin.

Additional information

Supported by the National Natural Science Foundation of China(Nos.21472067, 21672083), the Taishan Scholar Foundation, China(No.TS20151104 1) and the Startup Fund of University of Jinan, China(No.309-10004).

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Xu, G., Tang, Y. & Lin, W. Development of a Two-photon Ratiometric Fluorescent Probe for Glutathione and Its Applications in Living Cells. Chem. Res. Chin. Univ. 34, 523–527 (2018). https://doi.org/10.1007/s40242-018-8089-y

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  • DOI: https://doi.org/10.1007/s40242-018-8089-y

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