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Screening of Nutrients for Enrichment of Extracellular Pullulanase Production by Isolated Bacillus cereus KKSJ1981 Using Plackett–Burman Design

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Abstract

Current work intended to screen the different carbon, nitrogen sources and minerals which have a significant impact on the production of pullulanase by isolated Bacillus cereus KKSJ 1981 using Plackett–Burman design (PBD). Total 11 compounds (5 carbon sources, 3 nitrogen sources and 3 mineral salts) were screened by using 16 runs PBD. Each variable studied at 3 levels. The data were analyzed by first-order polynomial equation. The correlation coefficient was found to be 0.9876 and indicates the goodness of fit. Pareto chart of effects, normal probability plot and main effects plots were used to identify the significant variables and their level. It was observed that monosaccharide’s and dextran were found to be insignificant and remaining all variables found to be significant for production of pullulanase by isolated bacteria. Among all significant variables yeast extract has highest effect followed by soluble starch and MnSO4. This study reveals that yeast extract at low concentration, soluble starch and MnSO4 at higher level needed for the high titer of pullulanase production from B.cereus KKSJ 1981.

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Authors and Affiliations

  1. Hindu College of Pharmacy, Amaravathi Road, Guntur, Andhra Pradesh, 522002, India

    Jonna Siva Jyothi

  2. Sri Vishnu College of Pharmacy, Bhimavaram, Andhra Pradesh, 534202, India

    Emandi Hemalatha

  3. Department of Pharmacology, Koringa College of Pharmacy, Kakinada, Andhra Pradesh, 533461, India

    Tadikamalla Satish

  4. Narayana Pharmacy College, JNTUA, Chintareddy Palem, Nellore, Ananthapuramu, Andhra Pradesh, 524002, India

    Kadimpati Kishore Kumar

Authors
  1. Jonna Siva Jyothi
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  2. Emandi Hemalatha
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  3. Tadikamalla Satish
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  4. Kadimpati Kishore Kumar
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Contributions

KK and EH designed and envisaged the idea of the whole experiments. JS performed the tests and experiments, and TS designed the QBD and optimization studies and written the first version of the draft. KK and EH supervised the work and revised the manuscript.

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Correspondence to Kadimpati Kishore Kumar.

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Siva Jyothi, J., Hemalatha, E., Satish, T. et al. Screening of Nutrients for Enrichment of Extracellular Pullulanase Production by Isolated Bacillus cereus KKSJ1981 Using Plackett–Burman Design. Natl. Acad. Sci. Lett. 46, 233–238 (2023). https://doi.org/10.1007/s40009-023-01241-y

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  • DOI: https://doi.org/10.1007/s40009-023-01241-y

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