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Rapid, Efficient Direct and Indirect Regeneration Protocol of Dioscorea deltoidea Wall

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Abstract

The present study was explained about a direct and indirect regeneration protocol for an important wild medicinal plant Dioscorea deltoidea, a rich source of saponins and diosgenin. It was found that source of explant, basal medium composition, concentration of plant growth regulator and the photoperiod directly control morphogenetic responses. Out of all explants tested, only nodal segments performed better on the experimental growth media. Nodal segments initiated direct organogenesis when cultured on MS and RT (revised tobacco) medium supplemented with BAP and NAA or IBA. Growth medium supplemented with BAP (1.5 mg/l) and IBA (0.5 mg/l) induced maximum regeneration. Indirect roots regeneration was achieved on RT medium supplemented with IAA 1.0 mg/l and RT medium supplemented with BAP 1.0 mg/l and NAA 0.5 mg/l induced shoot regeneration through callus formed on MS medium containing 2, 4-D (2.0 mg/l).

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Acknowledgements

The authors are thankful to the Head, Department of Botany and Plant Physiology, CCS HAU, Hisar for providing the required research facilities.

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Authors and Affiliations

  1. Division of Crop Improvement, ICAR-Central Soil Salinity Research Institute, Karnal, Haryana, 132001, India

    Ashwani Kumar & Charu Lata

  2. CCS Haryana Agricultural University, Hisar, Haryana, 125004, India

    Ashwani Kumar, Suresh Chand Goyal, Pooja Dhansu & Jagdish Parshad

  3. ICAR-National Bureau of Plant Genetic Resources, New Delhi, 110012, India

    Neelam Sharma

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  1. Ashwani Kumar
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  2. Suresh Chand Goyal
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  3. Charu Lata
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  4. Neelam Sharma
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  5. Pooja Dhansu
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  6. Jagdish Parshad
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Correspondence to Ashwani Kumar.

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Kumar, A., Goyal, S.C., Lata, C. et al. Rapid, Efficient Direct and Indirect Regeneration Protocol of Dioscorea deltoidea Wall. Natl. Acad. Sci. Lett. 40, 237–240 (2017). https://doi.org/10.1007/s40009-017-0562-5

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  • DOI: https://doi.org/10.1007/s40009-017-0562-5

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