Abstract
Twenty-six eggplant genotypes of different colours were evaluated for their variations in total phenolics, total flavonoids, total monomeric anthocyanin and antioxidant activity. Antioxidant potentiality was determined using two in vitro assays, viz. cupric ion reducing antioxidant capacity and 2, 2-diphenyl-1-picrylhydrazyl (DPPH). Additionally, five colour attributes were evaluated. Among the eggplant genotypes, significant differences (p < 0.05) were observed with respect to antioxidant composition and antioxidant activity. Total phenolics and total flavonoids varied from 13.00 to 49.29 mg/100 g fresh weight (FW) and 5.30 to 28.70 mg/100 g FW, respectively. Chemometric tools such as principal component analysis (PCA) and agglomerative hierarchical clustering (AHC) were used to understand the possible classification of Indian eggplant genotypes based on their colour properties, antioxidant composition and antioxidant potentiality. PCA revealed that the first two components represented 80.68% of the total variability in the total variation. AHC classified genotypes into three main groups on the basis of the measured parameters. A white-coloured genotype was found to be the richer source of phenolics and flavonoids with high antioxidant activity.
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Akanitapichat P, Phraibung K, Nuchklang K, Prompitakkul S (2010) Antioxidant and hepatoprotective activities of five eggplant varieties. Food Chem Toxicol 48:3017–3021
Apak R, Güçlü K, Özyürek M, Karademir SE (2004) Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J Agric Food Chem 52:7970–7981
Azuma K, Ohyama A, Ippoushi K, Ichiyanagi T, Takeuchi A, Saito T, Fukuoka H (2008) Structures and antioxidant activity of anthocyanins in many accessions of eggplant and its related species. J Agric Food Chem 56:10154–10159
Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol 28:25–30
Cao G, Sofic E, Prior RL (1996) Antioxidant capacity of tea and common vegetables. J Agric Food Chem 44:3426–3431
Cushnie TT, Lamb AJ (2011) Recent advances in understanding the antibacterial properties of flavonoids. Int J Antimicrob Agents 38:99–107
Hanson PM, Yang RY, Tsou SC, Ledesma D, Engle L, Lee TC (2006) Diversity in eggplant (Solanum melongena) for superoxide scavenging activity, total phenolics, and ascorbic acid. J Food Compos Anal 19:594–600
Hair JFK, Anderson RE, Black WC, Tatham RL (2005) Multivariate data analysis. Pearson Prentice Hall, New Jersey
Kaneyuki T, Noda Y, Traber MG, Mori A, Packer L (1999) Superoxide anion and hydroxyl radical scavenging activities of vegetable extracts measured using electron spin resonance. Biochem Mol Biol Int 47:979–989
Kaur C, Nagal S, Nishad J, Kumar R (2014) Evaluating eggplant (Solanum melongena L) genotypes for bioactive properties: a chemometric approach. Food Res Int 60:205–211
Koley TK, Singh S, Khemariya P, Sarkar A, Kaur C, Chaurasia SNS, Naik PS (2014) Evaluation of bioactive properties of Indian carrot (Daucus carota L.): a chemometric approach. Food Res Int 60:76–85
Kwon YI, Apostolidis E, Shetty K (2008) In vitro studies of eggplant (Solanum melongena) phenolics as inhibitors of key enzymes relevant for type 2 diabetes and hypertension. Bioresour Technol 99:2981–2988
Matsubara K, Kaneyuki T, Miyake T, Mori M (2005) Antiangiogenic activity of nasunin, an antioxidant anthocyanin, in eggplant peels. J Agric Food Chem 53:6272–6275
NHB data base (2016) National horticulture data base, Government of India
Nijveldt RJ, Van Nood ELS, Van Hoorn DE, Boelens PG, Van Norren K, Van Leeuwen PA (2001) Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr 74:418–425
Nisha P, Nazar PA, Jayamurthy P (2009) A comparative study on antioxidant activities of different varieties of Solanum melongena. Food Chem Toxicol 47:2640–2644
Noda Y, Kaneyuki T, Igarashi K, Mori A, Packer L (2000) Antioxidant activity of nasunin, an anthocyanin in eggplant peels. Toxicol 148:119–123
Prohens J, Rodríguez-Burruezo A, Raigón MD, Nuez F (2007) Total phenolic concentration and browning susceptibility in a collection of different varietal types and hybrids of eggplant: implications for breeding for higher nutritional quality and reduced browning. J Am Soc Hortic Sci 132:638–646
Raigón MD, Rodríguez-Burruezo A, Prohens J (2010) Effects of organic and conventional cultivation methods on composition of eggplant fruits. J Agric Food Chem 58:6833–6840
Rathee P, Chaudhary H, Rathee S, Rathee D, Kumar V, Kohli K (2009) Mechanism of action of flavonoids as anti-inflammatory agents: a review. Inflamm Allergy Drug Targets 8:229–235
Ravishankar D, Rajora AK, Greco F, Osborn HM (2013) Flavonoids as prospective compounds for anti-cancer therapy. Int J Biochem Cell Biol 45:2821–2831
Sadilova E, Stintzing FC, Carle R (2006) Anthocyanins, colour and antioxidant properties of eggplant (Solanum melongena L.) and violet pepper (Capsicum annuum L.) peel extracts. Z Naturforsch C 61:527–535
Singh AP, Luthria D, Wilson T, Vorsa N, Singh V, Banuelos GS, Pasakdee S (2009) Polyphenols content and antioxidant capacity of eggplant pulp. Food Chem 114:955–961
Singleton VL, Orthofer R, Lamuela-Raventos RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Methods Enzymol 299:152–178
Stommel JR, Whitaker BD (2003) Phenolic acid content and composition of eggplant fruit in a germplasm core subset. J Am Soc Hortic Sci 128:704–710
Sudheesh S, Sandhya C, Koshy AS, Vijayalakshmi NR (1999) Antioxidant activity of flavonoids from Solanum melongena. Phytother Res 13:393–396
Tateyama C, Igarashi K (2006) Anthocyanin and chlorogenic acid contents of some selected eggplant (Solanum melongena L.) cultivars, and the radical scavenging activities of their extracts. J Jpn Soc Food Sci 53:218–224
Todaro A, Cimino F, Rapisarda P, Catalano AE, Barbagallo RN, Spagna G (2009) Recovery of anthocyanins from eggplant peel. Food Chem 114:434–439
Wrolstad RE, Durst RW, Lee J (2005) Tracking color and pigment changes in anthocyanin products. Trends Food Sci Technol 16:423–428
Yang RY (2006) Application of antioxidant activity analytical methods for studies on antioxidant activities of vegetables. Ph.D. Dissertation, Institute of Tropical Agriculture and International Cooperation of National Ping-tung University of Science and Technology, Taiwan
Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555–559
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This research is supported by the Indian Council of Agricultural Research, Government of India.
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TKK contributed to planning of experiment, analysis and manuscript writing; ST contributed to planning of experiment; AS contributed to statistical analysis; AG contributed to growing of plant in field and laboratory analysis; JN contributed to manuscript writing; and BS contributed to planning of experiment and manuscript writing
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Koley, T.K., Tiwari, S.K., Sarkar, A. et al. Antioxidant Potential of Indian Eggplant: Comparison Among White, Purple and Green Genotypes Using Chemometrics. Agric Res 8, 9–20 (2019). https://doi.org/10.1007/s40003-018-0347-1
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DOI: https://doi.org/10.1007/s40003-018-0347-1