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Antioxidant Potential of Indian Eggplant: Comparison Among White, Purple and Green Genotypes Using Chemometrics

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Abstract

Twenty-six eggplant genotypes of different colours were evaluated for their variations in total phenolics, total flavonoids, total monomeric anthocyanin and antioxidant activity. Antioxidant potentiality was determined using two in vitro assays, viz. cupric ion reducing antioxidant capacity and 2, 2-diphenyl-1-picrylhydrazyl (DPPH). Additionally, five colour attributes were evaluated. Among the eggplant genotypes, significant differences (p < 0.05) were observed with respect to antioxidant composition and antioxidant activity. Total phenolics and total flavonoids varied from 13.00 to 49.29 mg/100 g fresh weight (FW) and 5.30 to 28.70 mg/100 g FW, respectively. Chemometric tools such as principal component analysis (PCA) and agglomerative hierarchical clustering (AHC) were used to understand the possible classification of Indian eggplant genotypes based on their colour properties, antioxidant composition and antioxidant potentiality. PCA revealed that the first two components represented 80.68% of the total variability in the total variation. AHC classified genotypes into three main groups on the basis of the measured parameters. A white-coloured genotype was found to be the richer source of phenolics and flavonoids with high antioxidant activity.

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References

  1. Akanitapichat P, Phraibung K, Nuchklang K, Prompitakkul S (2010) Antioxidant and hepatoprotective activities of five eggplant varieties. Food Chem Toxicol 48:3017–3021

    Article  CAS  PubMed  Google Scholar 

  2. Apak R, Güçlü K, Özyürek M, Karademir SE (2004) Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J Agric Food Chem 52:7970–7981

    Article  CAS  PubMed  Google Scholar 

  3. Azuma K, Ohyama A, Ippoushi K, Ichiyanagi T, Takeuchi A, Saito T, Fukuoka H (2008) Structures and antioxidant activity of anthocyanins in many accessions of eggplant and its related species. J Agric Food Chem 56:10154–10159

    Article  CAS  PubMed  Google Scholar 

  4. Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol 28:25–30

    Article  CAS  Google Scholar 

  5. Cao G, Sofic E, Prior RL (1996) Antioxidant capacity of tea and common vegetables. J Agric Food Chem 44:3426–3431

    Article  CAS  Google Scholar 

  6. Cushnie TT, Lamb AJ (2011) Recent advances in understanding the antibacterial properties of flavonoids. Int J Antimicrob Agents 38:99–107

    Article  CAS  PubMed  Google Scholar 

  7. Hanson PM, Yang RY, Tsou SC, Ledesma D, Engle L, Lee TC (2006) Diversity in eggplant (Solanum melongena) for superoxide scavenging activity, total phenolics, and ascorbic acid. J Food Compos Anal 19:594–600

    Article  CAS  Google Scholar 

  8. Hair JFK, Anderson RE, Black WC, Tatham RL (2005) Multivariate data analysis. Pearson Prentice Hall, New Jersey

    Google Scholar 

  9. Kaneyuki T, Noda Y, Traber MG, Mori A, Packer L (1999) Superoxide anion and hydroxyl radical scavenging activities of vegetable extracts measured using electron spin resonance. Biochem Mol Biol Int 47:979–989

    CAS  PubMed  Google Scholar 

  10. Kaur C, Nagal S, Nishad J, Kumar R (2014) Evaluating eggplant (Solanum melongena L) genotypes for bioactive properties: a chemometric approach. Food Res Int 60:205–211

    Article  CAS  Google Scholar 

  11. Koley TK, Singh S, Khemariya P, Sarkar A, Kaur C, Chaurasia SNS, Naik PS (2014) Evaluation of bioactive properties of Indian carrot (Daucus carota L.): a chemometric approach. Food Res Int 60:76–85

    Article  CAS  Google Scholar 

  12. Kwon YI, Apostolidis E, Shetty K (2008) In vitro studies of eggplant (Solanum melongena) phenolics as inhibitors of key enzymes relevant for type 2 diabetes and hypertension. Bioresour Technol 99:2981–2988

    Article  CAS  PubMed  Google Scholar 

  13. Matsubara K, Kaneyuki T, Miyake T, Mori M (2005) Antiangiogenic activity of nasunin, an antioxidant anthocyanin, in eggplant peels. J Agric Food Chem 53:6272–6275

    Article  CAS  PubMed  Google Scholar 

  14. NHB data base (2016) National horticulture data base, Government of India

  15. Nijveldt RJ, Van Nood ELS, Van Hoorn DE, Boelens PG, Van Norren K, Van Leeuwen PA (2001) Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr 74:418–425

    Article  CAS  PubMed  Google Scholar 

  16. Nisha P, Nazar PA, Jayamurthy P (2009) A comparative study on antioxidant activities of different varieties of Solanum melongena. Food Chem Toxicol 47:2640–2644

    Article  CAS  PubMed  Google Scholar 

  17. Noda Y, Kaneyuki T, Igarashi K, Mori A, Packer L (2000) Antioxidant activity of nasunin, an anthocyanin in eggplant peels. Toxicol 148:119–123

    Article  CAS  Google Scholar 

  18. Prohens J, Rodríguez-Burruezo A, Raigón MD, Nuez F (2007) Total phenolic concentration and browning susceptibility in a collection of different varietal types and hybrids of eggplant: implications for breeding for higher nutritional quality and reduced browning. J Am Soc Hortic Sci 132:638–646

    Article  CAS  Google Scholar 

  19. Raigón MD, Rodríguez-Burruezo A, Prohens J (2010) Effects of organic and conventional cultivation methods on composition of eggplant fruits. J Agric Food Chem 58:6833–6840

    Article  CAS  PubMed  Google Scholar 

  20. Rathee P, Chaudhary H, Rathee S, Rathee D, Kumar V, Kohli K (2009) Mechanism of action of flavonoids as anti-inflammatory agents: a review. Inflamm Allergy Drug Targets 8:229–235

    Article  CAS  PubMed  Google Scholar 

  21. Ravishankar D, Rajora AK, Greco F, Osborn HM (2013) Flavonoids as prospective compounds for anti-cancer therapy. Int J Biochem Cell Biol 45:2821–2831

    Article  CAS  PubMed  Google Scholar 

  22. Sadilova E, Stintzing FC, Carle R (2006) Anthocyanins, colour and antioxidant properties of eggplant (Solanum melongena L.) and violet pepper (Capsicum annuum L.) peel extracts. Z Naturforsch C 61:527–535

    Article  CAS  PubMed  Google Scholar 

  23. Singh AP, Luthria D, Wilson T, Vorsa N, Singh V, Banuelos GS, Pasakdee S (2009) Polyphenols content and antioxidant capacity of eggplant pulp. Food Chem 114:955–961

    Article  CAS  Google Scholar 

  24. Singleton VL, Orthofer R, Lamuela-Raventos RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Methods Enzymol 299:152–178

    Article  CAS  Google Scholar 

  25. Stommel JR, Whitaker BD (2003) Phenolic acid content and composition of eggplant fruit in a germplasm core subset. J Am Soc Hortic Sci 128:704–710

    Article  CAS  Google Scholar 

  26. Sudheesh S, Sandhya C, Koshy AS, Vijayalakshmi NR (1999) Antioxidant activity of flavonoids from Solanum melongena. Phytother Res 13:393–396

    Article  CAS  PubMed  Google Scholar 

  27. Tateyama C, Igarashi K (2006) Anthocyanin and chlorogenic acid contents of some selected eggplant (Solanum melongena L.) cultivars, and the radical scavenging activities of their extracts. J Jpn Soc Food Sci 53:218–224

    Article  CAS  Google Scholar 

  28. Todaro A, Cimino F, Rapisarda P, Catalano AE, Barbagallo RN, Spagna G (2009) Recovery of anthocyanins from eggplant peel. Food Chem 114:434–439

    Article  CAS  Google Scholar 

  29. Wrolstad RE, Durst RW, Lee J (2005) Tracking color and pigment changes in anthocyanin products. Trends Food Sci Technol 16:423–428

    Article  CAS  Google Scholar 

  30. Yang RY (2006) Application of antioxidant activity analytical methods for studies on antioxidant activities of vegetables. Ph.D. Dissertation, Institute of Tropical Agriculture and International Cooperation of National Ping-tung University of Science and Technology, Taiwan

  31. Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555–559

    Article  CAS  Google Scholar 

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Acknowledgements

This research is supported by the Indian Council of Agricultural Research, Government of India.

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TKK contributed to planning of experiment, analysis and manuscript writing; ST contributed to planning of experiment; AS contributed to statistical analysis; AG contributed to growing of plant in field and laboratory analysis; JN contributed to manuscript writing; and BS contributed to planning of experiment and manuscript writing

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Correspondence to Tanmay Kumar Koley.

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Koley, T.K., Tiwari, S.K., Sarkar, A. et al. Antioxidant Potential of Indian Eggplant: Comparison Among White, Purple and Green Genotypes Using Chemometrics. Agric Res 8, 9–20 (2019). https://doi.org/10.1007/s40003-018-0347-1

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  • DOI: https://doi.org/10.1007/s40003-018-0347-1

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