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Anti-inflammatory and analgesic activities of ethyl acetate and petroleum ether fractions of Cassia auriculata Linn. leaves

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Abstract

The effect of petroleum ether fraction (50, 100 and 200 mg/kg) and ethyl acetate fraction (50, 100 and 200 mg/kg) of Cassia auriculata Linn. (Caesalpiniaceae) leaves was investigated in various experimental models of pain and inflammation. Anti-inflammatory activity of petroleum ether fraction and ethyl acetate fraction was studied using carrageenan induced paw edema and cotton pellet induced granuloma models in rats. Analgesic activity was studied in mice using tail immersion and hot plate models. Both petroleum ether fraction and ethyl acetate fraction significantly (P < 0.01) inhibited carrageenan induced paw edema and granuloma formation in cotton pellet induced granuloma model. Petroleum ether fraction and ethyl acetate fraction significantly (P < 0.01) increased latency to flick tail in tail immersion method and elevated the mean basal reaction time in hot plate method. Phytochemical analysis of petroleum ether fraction showed presence of steroids and ethyl acetate fraction showed presence of flavonoids and tannins. The ethyl acetate fraction was found to be more effective than petroleum ether fraction. Thus the anti-inflammatory and analgesic potential of C. auriculata leaves may be due to presence of active constituents like flavonoids and tannins present in petroleum ether fraction.

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Acknowledgement

The authors are very grateful to the management and Principal, PE Society’s Modern College of Pharmacy, Nigdi, Pune, India for providing required facilities of this work. Authors are also grateful to Dr. Diwakar, Director, Botanical Survey of India for authentification of plant material

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Correspondence to D. D. Bandawane.

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Mali, A.A., Bandawane, D.D. & Hivrale, M.G. Anti-inflammatory and analgesic activities of ethyl acetate and petroleum ether fractions of Cassia auriculata Linn. leaves. Orient Pharm Exp Med 13, 191–197 (2013). https://doi.org/10.1007/s13596-012-0099-6

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  • DOI: https://doi.org/10.1007/s13596-012-0099-6

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