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Genetic characterization of Lithuanian honeybee lines based on ISSR polymorphism

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Abstract

This study presents the first results from the selection and evaluation of inter-simple sequence repeat markers for the genetic assessment of honeybee lines developed in Lithuania and introduced subspecies. Two Lithuania-bred lines of Apis mellifera carnica were compared to those introduced from Czech Republic and Slovenia and also to a subspecies introduced from the Caucasus (Apis mellifera caucasica) and local Buckfast hybrids. The genetic constitution was assayed with four simple sequence primers: (ATG)5GA, (TCC)5GT, (AGAC)4GC and (GACA)4GT. Trinucleotide motif primers used in the bee DNA assays produced five to 10 fragments and tetranucleotide ones, six to seven. Primer (TCC)5GT generated a distinct A. m. caucasica-specific 800-bp fragment, which was also present in 40 % of the Buckfast individuals. A dendrogram based on the UPGMS method generated four sub-clusters. One of the Lithuania-bred A. m. carnica lines clustered apart from the other A. m. carnica lines. In a rooted phylogenetic tree, the group containing A. m. caucasica and Buckfast hybrids appeared well differentiated from the A. m. carnica lines.

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Acknowledgments

The authors kindly acknowledge the support of the Lithuanian Ministry of Agriculture provided to this study, as well as those who assisted in this project.

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Authors and Affiliations

  1. Institute of Agriculture, Lithuanian Research Centre for Agriculture and Forestry, 58344, Akademija, Kedainiai distr., Lithuania

    Violeta Ceksteryte, Vanda Paplauskiene & Diana Tamasauskiene

  2. Faculty of Natural Sciences, Vilnius University, M. K. Ciurlionio 21/27, 03101, Vilnius, Lithuania

    Izolda Pasakinskiene

  3. Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, 54333, Babtai, Kaunas reg., Lithuania

    Ingrida Mazeikiene

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  1. Violeta Ceksteryte
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  2. Vanda Paplauskiene
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  3. Diana Tamasauskiene
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  4. Izolda Pasakinskiene
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  5. Ingrida Mazeikiene
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Correspondence to Violeta Ceksteryte.

Additional information

Manuscript editor: Klaus Hartfelder

Caractérisation génétique des lignées d'abeilles de Lithuanie basée sur le polymorphisme ISSR

Lignées d'abeilles / distance génétique / empreinte génétique

Genetische Charakterisierung von Bienenlinien in Litauen basierend auf IRRS Polymorphismus

Schlüsselwörter: Honigbienen-Linien / DNA Fingerabdrücke / Genetische Distanzen

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Ceksteryte, V., Paplauskiene, V., Tamasauskiene, D. et al. Genetic characterization of Lithuanian honeybee lines based on ISSR polymorphism. Apidologie 43, 652–662 (2012). https://doi.org/10.1007/s13592-012-0140-2

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  • DOI: https://doi.org/10.1007/s13592-012-0140-2

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