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Construction of genetic linkage maps of ‘Fina Sodea’ clementine (Citrus clementina) and Byungkyul (C. platymamma), a Korean landrace, based on RAPD and SSR markers

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  • Genetics and Breeding
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Abstract

In this study, genetic linkage maps were constructed for ‘Fina Sodea’ clementine (Citrus clementina) and Byungkyul (C. platymamma) using 152 F1 clones as a mapping population. Reference-guided whole-genome sequencing, using next-generation sequencing technology, along with 82 randomly amplified polymorphic DNA markers and seven genomic simple-sequence repeat (gSSR) loci, mined 41 polymorphic gSSR loci from the direct comparative analysis. The generated linkage map for ‘Fina Sodea’ clementine contained 22 markers distributed into 10 linkage groups, with a total length of 417.7 cM and an average interval of 19.0 cM. The map for Byungkyul contained 57 markers distributed into 10 linkage groups, with a total length of 684.9 cM and a smaller average interval of 12.0 cM. Comparison of shared gSSR markers between the two maps identified collinearities, such as linkage groups B3 and C7 and groups B9 and C7. The newly developed gSSR markers greatly enrich the limited amount of gSSR markers in Citrus, providing markers that can be useful for comparative mapping and other genetic studies.

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Acknowledgements

This study was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Golden Seed Project (No. 213007-05-1-SBQ10), funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA).

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Authors and Affiliations

  1. Faculty of Bioscience and Industry, SARI, Jeju National University, Jeju, 63243, Korea

    Yali Chang, Eun-Ui Oh, Kyunguk Yi & Kwan Jeong Song

  2. Life Science Research Institute, Biomedic Co., Ltd, Bucheon, 14548, Republic of Korea

    Ho Bang Kim

  3. Research Institute for Subtropical Agriculture and Biotechnology, Jeju National University, Jeju, 63243, Republic of Korea

    Kwan Jeong Song

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  1. Yali Chang
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  2. Ho Bang Kim
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  3. Eun-Ui Oh
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  4. Kyunguk Yi
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Correspondence to Kwan Jeong Song.

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Chang, Y., Kim, H.B., Oh, EU. et al. Construction of genetic linkage maps of ‘Fina Sodea’ clementine (Citrus clementina) and Byungkyul (C. platymamma), a Korean landrace, based on RAPD and SSR markers. Hortic. Environ. Biotechnol. 59, 263–274 (2018). https://doi.org/10.1007/s13580-018-0021-3

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  • DOI: https://doi.org/10.1007/s13580-018-0021-3

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