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Bioconversion of waste glycerol for enhanced lipid accumulation in Trichosporon shinodae

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Abstract

Oleaginous yeast lipids have myriad of industrial applications that are gaining significant interest owing to shorter incubation, ability to use broad spectrum substrates, and quality lipids. However, the lipid content produced is low and need to enhance by optimization of varied parameters. In the present study, crude glycerol a by-product of biodiesel industry was supplemented to Trichosporon shinodae for lipid accumulation using central composite design (CCD) of response surface methodology (RSM). The developed quadratic model was found to be significant with the R2 value of 95.20% and adj. R2 value of 91.97%. An optimal lipid content of 49.85 ± 0.8% (w/w) was obtained using T. shinodae with 6.2% (v/v) inoculum volume, pH 3.6, C/N ratio 105, 1.52 (g/L) of MgSO4, and 4.55 mM FeSO4 in 120.72 h at 30 °C. Lipid composition from T. shinodae depicted the presence of linoleic acid (C18:2), oleic acid (C18:1), stearic acid (C18:0), palmitic acid (C18:0), myristic acid (C14:0), and lauric acids (C12:0), respectively. T. shinodae lipids have 61.1% (w/w) saturated fatty acids and unsaturated fatty acids proportion accord to 38.9% (w/w). Lipid composition of T. shinodae indicates that these lipids were suitable for synthesis of high value products like fuel additives, surfactants, detergents, and cleaning applications.

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Acknowledgements

Authors are grateful to the Director, Indian Institute of Technology, Kharagpur for all the support rendered in the completion of the work.

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Authors and Affiliations

  1. Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur, 721302, West Bengal, India

    S. P. Jeevan Kumar & Rintu Banerjee‬‬‬‬‬‬‬‬‬

  2. ICAR-Indian Institute of Seed Science, Kushmaur, Mau, 275103, U.P., India

    S. P. Jeevan Kumar

  3. ICAR-Directorate of Floricultural Research, Pune, 411036, Maharashtra, India

    S. P. Jeevan Kumar

  4. Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, 173234, H.P., India

    Vijay Kumar Garlapati

  5. Advanced Technology Development Centre, Indian Institute of Technology, Kharagpur, 721302, India

    Gujjala Lohit Kumar Srinivas

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Contributions

SPJK has conducted the experiments, analyzed the data, and drafted the paper. GVK analysed the RSM data and drafted the paper. RB conceived the research work, analysed, and edited the manuscript.

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Correspondence to Rintu Banerjee‬‬‬‬‬‬‬‬‬.

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Jeevan Kumar, S.P., Kumar Garlapati, V., Lohit Kumar Srinivas, G. et al. Bioconversion of waste glycerol for enhanced lipid accumulation in Trichosporon shinodae. Biomass Conv. Bioref. 13, 15401–15412 (2023). https://doi.org/10.1007/s13399-021-01799-x

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