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One-vessel saccharification and fermentation of pretreated sugarcane bagasse using a helical impeller bioreactor

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Abstract

The effect of Tween® 80 and the cellulase load, on the enzymatic hydrolysis of hydrothermally pretreated sugarcane bagasse (HPSB), was evaluated in shake flask experiments, using experimental design. The optimized conditions were further applied in a second set of shake flask experiments to study the effect of the biomass load. The overall optimum parameters, e.g., 6.9% Tween® 80, 15 FPU/g glucan, and 150 g/L (dry HPSB), were used in hydrolysis experiments carried out in a laboratory-scale bioreactor equipped with a helical impeller. After a 48 h reaction time, 60% of the HPSB glucan content was hydrolyzed into glucose. The same bioreactor and hydrolysis conditions were used for one-vessel saccharification and fermentation experiments as follows: 150 g/L (dry HPSB) was hydrolyzed at 50 °C and 150 rpm for either 24 or 48 h, followed by the bioreactor’s temperature and mixing decrease to 30 °C and 90 rpm for ethanol fermentation by Saccharomyces cerevisiae. Experiments resulted in ethanol yields of 48 or 52%, for hydrolysis time of 24 or 48 h, respectively, taking into account the HPSB glucan content. The best ethanol productivity, for the overall process of 0.51 g/L.h, was achieved for the 24 h hydrolysis time.

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Acknowledgments

We are grateful to Usina Santa Luzia–Odebrecht Agroindustrial, located in the state of Mato Grosso do Sul, Brazil, for providing the sugarcane bagasse. This work received financial support from Research and Projects Financing Agency (FINEP), The Brazilian Research Council (CNPq), and The Brazilian Federal Agency for Support and Evaluation of Graduate Education (CAPES).

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Authors and Affiliations

  1. Chemistry Institute, Federal University of Rio de Janeiro (UFRJ), Avenida Athos da Silveira Ramos 149, Bloco A, sala 539, Cidade Universitária CEP, Rio de Janeiro, RJ, 21941-909, Brazil

    Raul Alves de Oliveira & Elba Pinto da Silva Bon

  2. Embrapa Food Technology, Avenida das Américas 29501, Guaratiba, CEP, Rio de Janeiro, RJ, 23020-470, Brazil

    Leda Maria Fortes Gottschalk

  3. Chemical School, Federal University of Rio de Janeiro (UFRJ), Avenida Athos da Silveira Ramos 149, Bloco E, sala 211, Cidade Universitária CEP, Rio de Janeiro, RJ, 21941-909, Brazil

    Suely Pereira Freitas

  4. Chemistry Institute, Federal University of Rio de Janeiro (UFRJ), Av. Athos da Silveira Ramos, 149-, Bloco A-5° andar, sala 539, Rio de Janeiro, RJ, 21941-909, Brazil

    Elba Pinto da Silva Bon

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  1. Raul Alves de Oliveira
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  2. Leda Maria Fortes Gottschalk
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  3. Suely Pereira Freitas
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  4. Elba Pinto da Silva Bon
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Correspondence to Elba Pinto da Silva Bon.

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de Oliveira, R.A., Gottschalk, L.M.F., Freitas, S.P. et al. One-vessel saccharification and fermentation of pretreated sugarcane bagasse using a helical impeller bioreactor. Biomass Conv. Bioref. 8, 1–10 (2018). https://doi.org/10.1007/s13399-017-0272-8

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