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β-Adrenergic receptor gene expression in HIV-associated neurocognitive impairment and encephalitis: implications for MOR-1K subcellular localization

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Abstract

We previously reported that mRNA expression of the unique alternatively spliced OPRM1 isoform μ-opioid receptor-1K (MOR-1K), which exhibits excitatory cellular signaling, is elevated in HIV-infected individuals with combined neurocognitive impairment (NCI) and HIV encephalitis (HIVE). It has recently been shown that the β2-adrenergic receptor (β2-AR) chaperones MOR-1K, normally localized intracellularly, to the cell surface. Here, we found mRNA expression of the adrenoceptor beta 2 (ADRB2) gene is also elevated in NCI-HIVE individuals, as well as that β2-AR protein expression is elevated in HIV-1-infected primary human astrocytes treated with morphine, and discuss the implications for MOR-1K subcellular localization in this condition.

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Acknowledgments

We thank Kurt F. Hauser for use of the human tissue samples that were analyzed in this study as well as for use of laboratory space and equipment from which part of the data were generated. We are also most grateful to the individuals who donated the tissue. This work was supported by the National Institutes of Health (NIH)-National Institute on Drug Abuse (NIDA) grants F32 DA033898 to S.M.D. and R01 DA036154 to N.E.H. The human tissue provided by the National NeuroAIDS Tissue Consortium (NNTC) for this publication was made possible from NIH funding through the National Institute of Mental Health (NIMH) and National Institute of Neurological Disorders and Stroke (NINDS) by the following grants: Manhattan HIV Brain Bank: U01 MH083501, R24 MH59724; Texas NeuroAIDS Research Center: U01 MH083507, R24 NS45491; National Neurological AIDS Bank: 5U01 MH083500, NS38841; California NeuroAIDS Tissue Network: U01 MH083506, R24 MH59745; and Statistics and Data Coordinating Center: U01 MH083545, N01 MH32002. This publication’s contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH or the NNTC or endorsement by any other individuals.

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Correspondence to Nazira El-Hage.

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Dever, S.M., Rodriguez, M. & El-Hage, N. β-Adrenergic receptor gene expression in HIV-associated neurocognitive impairment and encephalitis: implications for MOR-1K subcellular localization. J. Neurovirol. 22, 866–870 (2016). https://doi.org/10.1007/s13365-016-0464-1

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  • DOI: https://doi.org/10.1007/s13365-016-0464-1

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