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Isolation and validation of eight microsatellite loci in a damselfly Ischnura senegalensis (Odonata: Coenagrionidae) by pyrosequencing a bead-enriched library

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Abstract

Eight microsatellite markers for the population genetics and evolutionary ecology of Ischnura senegalensis (Rambur 1842), which shows body color polymorphism in females, were developed using a streptavidin-bead enrichment library and pyrosequence by a next generation sequencer. The number of alleles per locus and effective number of alleles ranged from 3 to 11 and from 1.24 to 5.51, respectively. Observed and expected heterozygosities were 0.18–0.75 and 0.19–0.77, respectively. No linkage disequilibrium between loci was detected. One locus, IsenAC75, deviated significantly from the Hardy–Weinberg equilibrium, and the locus and additional two loci, IsenAC40 and IsenAC8, were suspected for the presence of null alleles. Altogether, these eight microsatellite loci are considered to be useful for population genetic analyses because of the high polymorphic status and independency.

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Acknowledgments

This study was supported by the Tropical Biosphere Research Center Project Grant, University of the Ryukyus, to MT and KY and by the Collaborative Research of Tropical Biosphere Research Center, University of the Ryukyus, to KS and NI.

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Correspondence to Takaki Kurita.

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Kurita, T., Aoyama, H., Saitoh, S. et al. Isolation and validation of eight microsatellite loci in a damselfly Ischnura senegalensis (Odonata: Coenagrionidae) by pyrosequencing a bead-enriched library. Appl Entomol Zool 49, 623–626 (2014). https://doi.org/10.1007/s13355-014-0292-9

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  • DOI: https://doi.org/10.1007/s13355-014-0292-9

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