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Phylogenetic analysis of B2L gene of Egyptian orf virus from naturally infected sheep

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Abstract

Orf is a viral disease caused by a parapoxvirus, affecting primarily sheep and goats and causes severe economic losses. In this study, a total of 500 sheep from a farm in El-Beheira Governorate, Egypt were examined during spring, 2014. Out of them, 30 sheep showed clinical signs of orf virus infection. The diseased sheep exhibited proliferative lesions on the lips and around the mouth. Polymerase chain reaction (PCR) was used for diagnosis of the disease. For genetic characterization of the Egyptian orf virus, the sequence of a major and highly immunogenic envelope protein gene (B2L gene) was identified and compared with the sequences available from different parts of the world. The virus was detected in 24 out of 30 collected samples (80 %) by PCR. Phylogenetic analyses of the Egyptian orf virus B2L gene showed close genetic relationship with Israel orf viruses those were identified in 2012. In conclusion, this study reports identification and genetic characterization of Egyptian orf virus in sheep in Egypt.

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References

  1. Ali MH, Ahmed MH, Tamam SM, Arafa A, Saad AA, Ali WF. Molecular characterization of orf virus isolated from sheep and goats in Egypt. Global Veterinaria. 2013;11:98–106.

    Google Scholar 

  2. Bouznach A, Hahn S, Stram Y, Menasherov S, Edery N, Shicaht N, et al. Case report: contagious ecthyma—deviations in the anatomical appearance of lesions in an outbreak in lambs in israel. Isr J Vet Med. 2013;68:246–51.

    Google Scholar 

  3. Delhon G, Tulman ER, Afonso CL, Lu Z, de la Concha-Bermejillo A, Lehmkuhl HD, et al. Genomes of the Parapoxviruses: orf virus and bovine papular stomatitis virus. J Virol. 2004;78:168–77.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  4. Gammon DB, Evans DH. The 3′-to-5′ exonuclease activity of vaccinia virus DNA polymerase is essential and plays a role in promoting virus genetic recombination. J Virol. 2009;83:4236–50.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  5. Guo J, Rasmussen J, Wunschmann A, de La Concha-Bermejillo A. Genetic characterization of orf viruses isolated from various ruminant species of a zoo. Vet Microbiol. 2004;99:81–92.

    Article  CAS  PubMed  Google Scholar 

  6. Haig DM, Mercer AA. Ovine diseases. Orf Vet Res. 1998;29:311–26.

    CAS  PubMed  Google Scholar 

  7. Inoshima Y, Morooka A, Sentsui H. Detection and diagnosis of parapoxvirus by the polymerase chain reaction. J Virol Methods. 2000;84:201–8.

    Article  CAS  PubMed  Google Scholar 

  8. Kottaridi C, Nomikou K, Teodori L, Savini G, Lelli R, Markoulatos P, et al. Phylogenetic correlation of Greek and Italian orf virus isolates based on VIR gene. Vet Microbiol. 2006;116:310–6.

    Article  CAS  PubMed  Google Scholar 

  9. Maan S, Kumar A, Batra K, Singh M, Nanda T, Ghosh A, et al. Isolation and molecular characterization of contagious pustular dermatitis virus from Rajasthan, India. Virusdisease. 2014;25:376–80.

    Article  PubMed Central  PubMed  Google Scholar 

  10. Mahmoud M, Abdelrahman K, Soliman H. Molecular and virological studies on contagious pustular dermatitis isolates from Egyptian sheep and goats. Res Vet Sci. 2010;89:290–4.

    Article  PubMed  Google Scholar 

  11. Mazur C, Machado RD. Detection of contagious pustular dermatitis virus of goats in a severe outbreak. Vet Rec. 1989;125:419–20.

    Article  CAS  PubMed  Google Scholar 

  12. Oem JK, Roh IS, Lee KH, Lee KK, Kim HR, Jean YH, et al. Phylogenetic analysis and characterization of Korean orf virus from dairy goats: case report. Virol J. 2009;6:167.

    Article  PubMed Central  PubMed  Google Scholar 

  13. Radostits OM, Guy CC, Hinchcliff KW, Constable DD. Veterinary medicine. Philadelphia: Saunders; 2007. p. 1418–21.

    Google Scholar 

  14. Sullivan JT, Fleming SB, Robinson AJ. Identification and characterization of an orf homologue of the vaccinia virus gene encoding the major envelope antigen p37K. Virology. 1994;202:968–73.

    Article  CAS  PubMed  Google Scholar 

  15. Viljoen GJ, Nel LH, Crowther JR. Molecular diagnostic PCR handbook, IAEA-FAO. Dordrecht: Springer; 2005.

    Google Scholar 

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Correspondence to Mohamed El-Tholoth.

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El-Tholoth, M., Elnaker, Y.F. & Shiha, G. Phylogenetic analysis of B2L gene of Egyptian orf virus from naturally infected sheep. VirusDis. 26, 147–150 (2015). https://doi.org/10.1007/s13337-015-0264-x

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  • DOI: https://doi.org/10.1007/s13337-015-0264-x

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