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Genomic analysis of type strain Paenibacillus aceti L14T, a highly efficient producer of pyrazines

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Abstract

Paenibacillus aceti L14T (CGMCC 1.15420 = JCM 31170) is a novel species isolated from the solid-state acetic acid fermentation culture of traditional Chinese vinegar. The strain is able to biosynthesize the pyrazines, including 2,3-diisobutylpyrazine, 2-isobutyl-3-methylpyrazine and 1-(5-isobutyl-2-pyrazinyl)-1-propanone. Genome sequencing of L14 was performed to gain insights into the genetic elements involved in the biosynthesis of pyrazines. The genome of L14 contains 5,611,962 bp with a GC content of 47.92 mol%, 5147 protein coding genes, 92 tRNAs, 20 rRNAs and four sRNAs. The strain L14 also contains complete biosynthetic pathways of valine, leucine and isoleucine, and contains genes for encoding threonine dehydratase and ketol-acid reductoisomerase. This genome sequence provides a basis for elucidating the possible mechanism for the biosynthesis of pyrazines.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (grant no. 31271924).

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Authors and Affiliations

  1. College of Food Science, South China Agricultural University, Wushan Road, Tianhe District, Guangzhou, 510642, China

    Pan Li & Bing Du

  2. Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China

    Pan Li, Xing Gan & Lixin Luo

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  1. Pan Li
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  2. Xing Gan
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  3. Lixin Luo
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  4. Bing Du
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Correspondence to Lixin Luo or Bing Du.

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Li, P., Gan, X., Luo, L. et al. Genomic analysis of type strain Paenibacillus aceti L14T, a highly efficient producer of pyrazines. Ann Microbiol 67, 391–393 (2017). https://doi.org/10.1007/s13213-017-1267-1

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  • DOI: https://doi.org/10.1007/s13213-017-1267-1

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