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Taxonomy of mycelial actinobacteria isolated from Saharan soils and their efficiency to reduce aflatoxin B1 content in a solid-based medium

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Abstract

Aflatoxin B1 (AFB1) is a carcinogenic compound produced by filamentous fungi. In order to reduce AFB1 occurrence in foodstuffs, 13 strains of mycelial actinobacteria were tested in vitro for the efficacy to reduce AFB1 content; all were isolated from the Saharan soils of Algeria. Firstly, morphological study and molecular analysis, based on the 16S rRNA gene, indicated that these strains belong to Actinomadura, Nocardiopsis, Nonomuraea, Saccharothrix and Streptomyces genera. Secondly, each strain’s efficacy to reduce pure AFB1 content was studied in ISP2-medium. After a 4-day incubation at 30°C on AFB1-supplemented medium (5 ppm of AFB1), AFB1 was extracted and quantified. AFB1 content was reduced by all strains (42.9–97.6%). The three most efficient reducers (94.9–97.6%) were two strains belonging to the genus Streptomyces and one to the genus Saccharothrix. Among the latter, strains ACD6 and ABH19 showed no adsorption mechanism involved, suggesting a potential degradation mechanism. These findings led us to suggest that these actinobacterial strains could be used as decontamination treatments for the reduction of AFB1 content.

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Acknowledgments

The authors gratefully acknowledge support for this work from the “Comité mixte Franco-Algérien de l’Accord Programme TASSILI (PHC No. 15 MDU 932; Campus France: 33000WC)”.

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Authors and Affiliations

  1. Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure, Kouba, Alger, Algeria

    Abdelhadi Lahoum, Noureddine Bouras & Nasserdine Sabaou

  2. Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre, Université de Ghardaïa, BP 455, Ghardaïa, 47000, Algeria

    Noureddine Bouras

  3. Laboratoire de Génie Chimique, LGC, Université de Toulouse, UMR 5503 (CNRS/INPT/UPS), Toulouse, France

    Carol Verheecke-Vaessen & Florence Mathieu

  4. Applied Mycology Group, AgriFood Theme, Cranfield University, Cranfield, Bedford, MK43 0AL, UK

    Carol Verheecke-Vaessen

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  1. Abdelhadi Lahoum
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  2. Carol Verheecke-Vaessen
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Correspondence to Nasserdine Sabaou.

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Fig. S1

HPLC chromatograms showing AFB1 peaks. a AFB1 peak in uninoculated AFB1-supplemented medium b AFB1 peak after inoculation by the strain Saccharothrix sp. ABH25. The arrow indicates the emerged peak (DOC 56 kb)

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Lahoum, A., Verheecke-Vaessen, C., Bouras, N. et al. Taxonomy of mycelial actinobacteria isolated from Saharan soils and their efficiency to reduce aflatoxin B1 content in a solid-based medium. Ann Microbiol 67, 231–237 (2017). https://doi.org/10.1007/s13213-017-1253-7

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