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Identification of Potential Allergens of Atractylodes japonica and Addition of Panels for Allergic Diseases

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Abstract

The use of herbal medicines is increasing significantly worldwide. However, studies on side effects such as allergic reactions of herbal medicines are lacking. To provide an effective prescription, a diagnostic test to determine whether an allergic reaction is induced in the patient must be preceded. In this study, the purpose of this study is to investigate whether Atractylodes japonica extract, used as herbal medicine, binds to human serum IgE and induces an allergic reaction as an immune response complex, and to add a panel of allergens to the Allergy Q kit. First, using the Allergy-Q test kit, 253 sera samples were screened and 4 samples each from positive and negative serogroups were randomly selected. AJ extracts were separated by size through sodium dodecyl sulfate–polyacrylamide gel electrophoresis and stained with Coomassie blue dye. We then used Western blotting and in-gel digest to identify proteins that bind to human serum IgE, and predicted six proteins, including putative chitinases, proteasomes, and hypothetical proteins, using MASCOT program matching. Consequently, we demonstrated the potential of AJ as an allergen by confirming the sensitivity between AJ-derived proteins and human IgE.

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Data Availability

All data presented in this study are available from the corresponding author, upon reasonable request.

References

  1. McConnell, T.H.: The Nature of Disease: Pathology for the Health Professions. Lippincott Williams & Wilkins, Philadelphia (2007)

    Google Scholar 

  2. Sathe, S.K., Liu, C., Zaffran, V.D.: Food allergy. Annu. Rev. Food Sci. Technol. 7, 191–220 (2016)

    Article  CAS  PubMed  Google Scholar 

  3. Valenta, R., Hochwallner, H., Linhart, B., Pahr, S.: Food allergies: the basics. Gastroenterology 148, 1120-1131.e1124 (2015)

    Article  CAS  PubMed  Google Scholar 

  4. Bernaldo de Quiros, E., Seoane-Reula, E., Alonso-Lebrero, E., Pion, M., Correa-Rocha, R.: The role of regulatory T cells in the acquisition of tolerance to food allergens in children. Allergol. Immunopathol. 46, 612–618 (2018)

    Article  CAS  Google Scholar 

  5. Cardona, V., Ansotegui, I.J., Ebisawa, M., El-Gamal, Y., Fernandez Rivas, M., Fineman, S., Geller, M., Gonzalez-Estrada, A., Greenberger, P.A., Sanchez Borges, M., Senna, G., Sheikh, A., Tanno, L.K., Thong, B.Y., Turner, P.J., Worm, M.: World allergy organization anaphylaxis guidance 2020. World Allergy Organ. J. 13, 100472 (2020)

    Article  PubMed  PubMed Central  Google Scholar 

  6. Sampson, H.A.: Anaphylaxis and emergency treatment. Pediatrics 111, 1601–1608 (2003)

    Article  PubMed  Google Scholar 

  7. Jimenez-Rodriguez, T.W., Garcia-Neuer, M., Alenazy, L.A., Castells, M.: Anaphylaxis in the 21st century: phenotypes, endotypes, and biomarkers. J. Asthma Allergy 11, 121–142 (2018)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Sigerist, H.E.: A History of Medicine. Oxford University Press, Oxford (1987)

    Google Scholar 

  9. Kang, E.-M., Jeong, C.-H., Shim, K.-H.: Functional properties of Korean Atractylodes japonica Koidz. Korean J. Post-Harvest Sci. Technol. Agric. Prod. 8, 86–91 (2001)

    Google Scholar 

  10. Li, J., You, W.: Analysis of nuclear types in chromosome of Japanese Atractylodes. J. Shenyang Agric. Univ. 18, 75–77 (1987)

    Google Scholar 

  11. Jeong, S.-I., Kim, S.-Y., Kim, S.-J., Hwang, B.-S., Kwon, T.-H., Yu, K.-Y., Hang, S.-H., Suzuki, K., Kim, K.-J.: Antibacterial activity of phytochemicals isolated from Atractylodes japonica against methicillin-resistant Staphylococcus aureus. Molecules (Basel, Switzerland) 15, 7395–7402 (2010)

    Article  CAS  PubMed  Google Scholar 

  12. Satoh, K., Nagai, F., Ushiyama, K., Kano, I.: Specific inhibition of Na+, K+-ATPase activity by atractylon, a major component of Byaku-jutsu, by interaction with enzyme in the E2 state. Biochem. Pharmacol. 51, 339–343 (1996)

    Article  CAS  PubMed  Google Scholar 

  13. Kitajima, J., Kamoshita, A., Ishikawa, T., Takano, A., Fukuda, T., Isoda, S., Ida, Y.: Glycosides of Atractylodes japonica. Chem. Pharm. Bull. 51, 152–157 (2003)

    Article  CAS  Google Scholar 

  14. Hong, M.H., Kim, J.H., Bae, H., Lee, N.Y., Shin, Y.C., Kim, S.H., Ko, S.G.: Atractylodes japonica Koidzumi inhibits the production of proinflammatory cytokines through inhibition of the NF-kappaB/IkappaB signal pathway in HMC-1 human mast cells. Arch. Pharmacal Res. 33, 843–851 (2010)

    Article  CAS  Google Scholar 

  15. Yu, K.W., Kiyohara, H., Matsumoto, T., Yang, H.C., Yamada, H.: Intestinal immune system modulating polysaccharides from rhizomes of Atractylodes lancea. Planta Med. 64, 714–719 (1998)

    Article  CAS  PubMed  Google Scholar 

  16. Kiso, Y., Tohkin, M., Hikino, H.: Antihepatotoxic principles of Atractylodes rhizomes. J. Nat. Prod. 46, 651–654 (1983)

    Article  CAS  PubMed  Google Scholar 

  17. Lin, A.X., Chan, G., Hu, Y., Ouyang, D., Ung, C.O.L., Shi, L., Hu, H.: Internationalization of traditional Chinese medicine: current international market. Int Chall. Prospect. Suggest. 13, 9 (2018)

    Google Scholar 

  18. Agbabiaka, T.B., Pittler, M.H., Wider, B., Ernst, E.: Serenoa repens (saw palmetto): a systematic review of adverse events. Drug Saf. 32, 637–647 (2009)

    Article  PubMed  Google Scholar 

  19. Ansotegui, I.J., Melioli, G., Canonica, G.W., Caraballo, L., Villa, E., Ebisawa, M., Passalacqua, G., Savi, E., Ebo, D., Gómez, R.M., Luengo Sánchez, O., Oppenheimer, J.J., Jensen-Jarolim, E., Fischer, D.A., Haahtela, T., Antila, M., Bousquet, J.J., Cardona, V., Chiang, W.C., Demoly, P.M., DuBuske, L.M., Ferrer Puga, M., Gerth van Wijk, R., González Díaz, S.N., Gonzalez-Estrada, A., Jares, E., Kalpaklioğlu, A.F., Kase Tanno, L., Kowalski, M.L., Ledford, D.K., Monge Ortega, O.P., Morais Almeida, M., Pfaar, O., Poulsen, L.K., Pawankar, R., Renz, H.E., Romano, A.G., Rosário Filho, N.A., Rosenwasser, L., Sánchez Borges, M.A., Scala, E., Senna, G.E., Sisul, J.C., Tang, M.L.K., Thong, B.Y., Valenta, R., Wood, R.A., Zuberbier, T.: IgE allergy diagnostics and other relevant tests in allergy, a World Allergy Organization position paper. World Allergy Organ. J. 13, 100080 (2020)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Lee, J.H., Park, H.J., Park, K.H., Jeong, K.Y., Park, J.W.: Performance of the PROTIA™ Allergy-Q® system in the detection of allergen-specific IgE: a comparison with the ImmunoCAP® system. Allergy Asthma Immunol. Res. 7, 565–572 (2015)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. An, E.-J., Kim, K.-H., Lee, I.-S., Park, J.Y., Kim, Y., Jung, W.S., Kwon, D., Jang, H.-J.: Identification of possibility of Glycyrrhiza uralensis as an allergen by protein analysis. BioChip J. 12, 75–82 (2018)

    Article  CAS  Google Scholar 

  22. Kim, K.-H., Park, J.Y., Lee, I.-S., Kim, Y., Jang, H.-J.: Proteins derived from Prunus armeniaca kernel are possible to cause Immunoglobulin E reactivity in human sera. Mol. Cell. Toxicol. 13, 213–220 (2017)

    Article  CAS  Google Scholar 

  23. Park, J., Lee, I.-S., Kim, Y., An, E.-J., Jung, W.S., Jang, H.-J.: Proteins derived from Cnidium officinale Makino react with serum IgE of allergic patients and stimulate ERK/NF-kB activation in human mast cell line HMC-1 cells. BioChip J. 15, 135–143 (2021)

    Article  CAS  Google Scholar 

  24. Kim, Y., Chung, W.S., Jang, H.-J.: Proteins isolated of Pueraria Radix possible to cause allergenic react with immunoglobulin E in human sera. Mol. Cell. Toxicol. 14, 233–239 (2018)

    Article  CAS  Google Scholar 

  25. Kim, K.-H., Park, J.Y., Lee, I.-S., Lim, K.J., Jung, W.S., Jang, H.-J.: Isolated protein of Astragalus membranaceus acts as an allergen by binding human immunoglobulin E on human sera. BioChip J. 10, 95–102 (2016)

    Article  CAS  Google Scholar 

  26. Geneva, S.: WHO guidelines on safety monitoring of herbal medicines in pharmacovigilance systems. [Google Scholar] (2004)

  27. Jordan, S.A., Cunningham, D.G., Marles, R.J.: Assessment of herbal medicinal products: challenges, and opportunities to increase the knowledge base for safety assessment. Toxicol. Appl. Pharmacol. 243, 198–216 (2010)

    Article  CAS  PubMed  Google Scholar 

  28. Daniele, C., Thompson Coon, J., Pittler, M.H., Ernst, E.: Vitex agnus castus: a systematic review of adverse events. Drug Saf. 28, 319–332 (2005)

    Article  PubMed  Google Scholar 

  29. Daniele, C., Mazzanti, G., Pittler, M.H., Ernst, E.: Adverse-event profile of Crataegus spp.: a systematic review. Drug Saf. 29, 523–535 (2006)

    Article  CAS  PubMed  Google Scholar 

  30. Meincke, R., Pokladnikova, J., Straznicka, J.: Allergy-like immediate reactions with herbal medicines in children: a retrospective study using data from VigiBase(®). 28, 668–674 (2017)

  31. 정의민, 한의학 고전 문헌과 현대 약리 연구에서 보고된백출의 효능에 관한 비교연구 () 白朮

  32. 김송희, 박용기, 백출(白朮)의 콜라겐 유도 관절염 마우스에서의 관절염 개선 효과 연구. 대한본초학회지(본초분과학회지). 27, 1–6 (2012)

  33. Yamahara, J., Matsuda, H., Huang, Q., Li, Y., Fujimura, H.: Intestinal motility enhancing effect of Atractylodes lancea rhizome. J. Ethnopharmacol. 29, 341–344 (1990)

    Article  CAS  PubMed  Google Scholar 

  34. Sakurai, T., Sugawara, H., Saito, K.-I., Kano, Y.: Effects of the acetylene compound from Atractylodes rhizome on experimental gastric ulcers induced by active oxygen species. Biol. Pharm. Bull. 17, 1364–1368 (1994)

    Article  CAS  PubMed  Google Scholar 

  35. Wang, J.-H., Bose, S., Kim, H.-G., Han, K.-S., Kim, H.: Fermented Rhizoma Atractylodis macrocephalae alleviates high fat diet-induced obesity in association with regulation of intestinal permeability and microbiota in rats. Sci. Rep. 5, 1–10 (2015)

    Google Scholar 

  36. Han, Y., Jung, H.W., Park, Y.-K.: The roots of Atractylodes japonica Koidzumi promote adipogenic differentiation via activation of the insulin signaling pathway in 3T3-L1 cells. BMC Complement. Altern. Med. 12, 1–7 (2012)

    Article  Google Scholar 

  37. Muroi, M., Tanaka, K., Kimura, I., Kimura, M.: β-Eudesmol (a main component of Atractylodes lancea)-induced potentiation of depolarizing neuromuscular blockade in diaphragm muscles of normal and diabetic mice. Jpn. J. Pharmacol. 50, 69–71 (1989)

    Article  CAS  PubMed  Google Scholar 

  38. Chen, Y., Wu, Y., Wang, H., Gao, K.: A new 9-nor-atractylodin from Atractylodes lancea and the antibacterial activity of the atractylodin derivatives. Fitoterapia 83, 199–203 (2012)

    Article  CAS  PubMed  Google Scholar 

  39. Choi, S.W., Lee, K.S., Lee, J.H., Kang, H.J., Lee, M.J., Kim, H.Y., Park, K.I., Kim, S.L., Shin, H.K., Seo, W.D.: Suppression of Akt-HIF-1α signaling axis by diacetyl atractylodiol inhibits hypoxia-induced angiogenesis. BMB Rep. 49, 508–513 (2016)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  40. Ye, Y., Chou, G.-X., Wang, H., Chu, J.-H., Fong, W.-F., Yu, Z.-L.: Effects of sesquiterpenes isolated from largehead atractylodes rhizome on growth, migration, and differentiation of B16 melanoma cells. Integr. Cancer Ther. 10, 92–100 (2011)

    Article  Google Scholar 

  41. Li, X., Liu, F., Li, Z., Ye, N., Huang, C., Yuan, X.: Atractylodes macrocephala polysaccharides induces mitochondrial-mediated apoptosis in glioma C6 cells. Int. J. Biol. Macromol. 66, 108–112 (2014)

    Article  CAS  PubMed  Google Scholar 

  42. Woodfolk, J.A., Commins, S.P., Schuyler, A.J., Erwin, E.A., Platts-Mills, T.A.E.: Allergens, sources, particles, and molecules: why do we make IgE responses? Allergol. Int. 64, 295–303 (2015)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  43. Masilamani, M., Commins, S., Shreffler, W.: Determinants of food allergy. Immunol. Allergy Clin. North Am. 32, 11–33 (2012)

    Article  PubMed  PubMed Central  Google Scholar 

  44. Zukiewicz-Sobczak, W.A., Wróblewska, P., Adamczuk, P., Kopczyński, P.: Causes, symptoms and prevention of food allergy. Postepy Dermatol. Alergol. 30, 113–116 (2013)

    Article  PubMed  PubMed Central  Google Scholar 

  45. Nowak-Węgrzyn, A., Katz, Y., Mehr, S.S., Koletzko, S.: Non-IgE-mediated gastrointestinal food allergy. J. Allergy Clin. Immunol. 135, 1114–1124 (2015)

    Article  PubMed  Google Scholar 

  46. Sanz, M.L., de Las Marinas, M.D., Fernández, J., Gamboa, P.M.: Lupin allergy: a hidden killer in the home. Clin. Exp. Allergy 40, 1461–1466 (2010)

    Article  CAS  PubMed  Google Scholar 

  47. Boyce, J.A., Assa’ad, A., Burks, A.W., Jones, S.M., Sampson, H.A., Wood, R.A., Plaut, M., Cooper, S.F., Fenton, M.J., Arshad, S.H., Bahna, S.L., Beck, L.A., Byrd-Bredbenner, C., Camargo, C.A., Jr., Eichenfield, L., Furuta, G.T., Hanifin, J.M., Jones, C., Kraft, M., Levy, B.D., Lieberman, P., Luccioli, S., McCall, K.M., Schneider, L.C., Simon, R.A., Simons, F.E., Teach, S.J., Yawn, B.P., Schwaninger, J.M.: Guidelines for the diagnosis and management of food allergy in the United States: report of the NIAID-sponsored expert panel. J. Allergy Clin. Immunol. 126, S1-58 (2010)

    Article  PubMed  PubMed Central  Google Scholar 

  48. Iorio, R.A., Del Duca, S., Calamelli, E., Pula, C., Lodolini, M., Scamardella, F., Pession, A., Ricci, G.: Citrus allergy from pollen to clinical symptoms. PLoS ONE 8, e53680 (2013)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  49. Jee, W., Lee, S.H., Ko, H.M., Jung, J.H.: Anti-obesity effect of polygalin C isolated from Polygala japonica Houtt. via suppression of the adipogenic and lipogenic factors in 3T3-L1 adipocytes. 22 (2021)

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Acknowledgements

This research was supported by a Grant from the Traditional Korean Medicine R&D program funded by the Ministry of Health & Welfare through the Korea Health Industry Development Institute (KHIDI) (HI19C0544 and HF20C0030). Conceptualization, WJ and H-JJ; methodology, WJ; investigation, WJ and HMK; writing—original draft preparation, WJ; writing—review and editing, WJ, H-JJ, SWK and WSJ. All authors have read and agreed to the published version of the manuscript. We thank for members of Jang laboratory for active discussion.

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Jee, W., Ko, H.M., Kwon, S.W. et al. Identification of Potential Allergens of Atractylodes japonica and Addition of Panels for Allergic Diseases. BioChip J 17, 104–111 (2023). https://doi.org/10.1007/s13206-022-00094-9

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