Abstract
A halophilic bacterial consortium was enriched from Red Sea saline water and sediment samples collected from Abhor, Jeddah, Saudi Arabia. The consortium potentially degraded different low (above 90% for phenanthrene and fluorene) and high (69 ± 1.4 and 56 ± 1.8% at 50 and 100 mg/L of pyrene) molecular weight polycyclic aromatic hydrocarbons (PAHs) at different concentrations under saline condition (40 g/L NaCl concentration). The cell hydrophobicity (91° ± 1°) and biosurfactant production (30 mN/m) confirmed potential bacterial cell interaction with PAHs to facilitate biodegradation process. Co-metabolic study with phenanthrene as co-substrate during pyrene degradation recorded 90% degradation in 12 days. The consortium in continuous stirred tank reactor with petroleum refinery wastewater showed complete and 90% degradation of low and high molecular weight PAHs, respectively. The reactor study also revealed 94 ± 1.8% chemical oxygen demand removal by the halophilic consortium under saline condition (40 g/L NaCl concentration). The halophilic bacterial strains present in the consortium were identified as Ochrobactrum halosaudis strain CEES1 (KX377976), Stenotrophomonas maltophilia strain CEES2 (KX377977), Achromobacter xylosoxidans strain CEES3 (KX377978) and Mesorhizobium halosaudis strain CEES4 (KX377979). Thus, the promising halophilic consortium was highly recommended to be employed in petroleum saline wastewater treatment process.
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References
Arulazhagan P, Vasudevan N (2009) Role of moderately halophilic bacterial consortium in biodegradation of polyaromatic hydrocarbons. Mar Pollut Bull 58:256–262. https://doi.org/10.1016/j.marpolbul.2008.09.017
Arulazhagan P, Vasudevan N (2011) Biodegradation of polycyclic aromatic hydrocarbons by a halotolerant bacterial strain Ochrobactrum sp. VA1. Mar Pollut Bull 62:388–394. https://doi.org/10.1016/j.marpolbul.2010.09.020
Arulazhagan P, Yeom IT, Sivaraman C, Srikanth M, Rajesh Banu J (2013) Role of nutrients on biodegradation of 1,4-dioxane by a bacterial consortium enriched from industrial sludge. Adv Environ Biol 7:2081–2090
Arulazhagan P, Sivaraman C, Adish Kumar S, Aslam M, Rajesh Banu J (2014) Co-metabolic degradation of benzo(e)pyrene by halophilic bacterial consortium at different saline conditions. J Environ Biol 35:445–452
Atagana HI, Haynes RJ, Wallis FM (2003) Optimization of soil physical and chemical conditions for the bioremediation of creosote-contaminated soil. Biodegradation 14:297–307
Bonfa MRL, Grossman MJ, Mellado E, Durrant LR (2011) Biodegradation of aromatic hydrocarbons by haloarchaea and their use for the reduction of the chemical oxygen demand of hypersaline petroleum produced water. Chemosphere 84(11):1671–1676. https://doi.org/10.1016/j.chemosphere.2011.05.005
Chandrasekaran S, Pugazhendi A, Banu RJ, Ismail IMI, Qari HA (2018) Biodegradation of phenol by a moderately halophilic bacterial consortium. Environ Prog Sustain Energy. https://doi.org/10.1002/ep.12834 (Article online)
Dastgheib SMM, Amoozegar MA, Khajeh K, Shavandi M, Ventosa A (2012) Biodegradation of polycyclic aromatic hydrocarbons by a halophilic microbial consortium. Appl Microbiol Biotechnol 95(3):789–798. https://doi.org/10.1007/s00253-011-3706-4
Elgh-Dalgren K, Arwidsson Z, Ribé V, Waara S, von Kronhelm T, van Hees PAW (2011) Bioremediation of a soil industrially contaminated by wood preservatives-degradation of polycyclic aromatic hydrocarbons and monitoring of coupled arsenic translocation. Water Air Soil Pollut 214(1–4):275–285. https://doi.org/10.1007/s11270-010-0422-0
Fingas MF (2013) Modeling oil and petroleum evaporation. J Pet Sci Res 2(3):104–115
Gao S, Seo JS, Wang J, Keum YS, Li J, Li QX (2013) Multiple degradation pathways of phenanthrene by Stenotrophomonas maltophilia C6. Int Biodeterior Biodegrad 79:98–104. https://doi.org/10.1016/j.ibiod.2013.01.012
Ghosal D, Ghosh S, Dutta TK, Ahn Y (2016) Current state of knowledge in microbial degradation of polycyclic aromatic hydrocarbons (PAHs): a review. Front Microbiol 7:1369. https://doi.org/10.3389/fmicb.2016.01369
Gomes MB, Gonzales-Limache EE, Sousa STP, Dellagnezze BM, Sartoratto A et al (2018) Exploring the potential of halophilic bacteria from oil terminal environments for biosurfactant production and hydrocarbon degradation under high-salinity conditions. Int Biodeterior Biodegrad 126:231–242. https://doi.org/10.1016/j.ibiod.2016.08.014
Guo G, He F, Tian F, Huang Y, Wang H (2016) Effect of salt contents on enzymatic activities and halophilic microbial community structure during phenanthrene degradation. Int Biodeterior Biodegrad 110:8–15. https://doi.org/10.1016/j.ibiod.2016.02.007
Harms H, Smith KEC, Wick LY (2010) Microorganism–hydrophobic compound interactions. In: Timmis KN, McGenity TJ, van der Meer JR, de Lorenzo V (eds) Handbook of hydrocarbon and lipid microbiology. Springer, Berlin, pp 1479–1490
Johnsen AR, Wick LY, Harms H (2005) Principles of microbial PAH degradation in soil. Environ Pollut 133:71–84. https://doi.org/10.1016/j.envpol.2004.04.015
Kiyohara H, Nagao K, Yana K (1982) Rapid screen for bacteria degrading water insoluble, solid hydrocarbons on agar plates. Appl Environ Microbiol 43:454–457
Lamichhane S, Bal Krishna KC, Sarukkalige R (2017) Surfactant-enhanced remediation of polycyclic aromatic hydrocarbons: a review. J Environ Manag 199:46–61
Lefebvre O, Moletta R (2006) Treatment of organic pollution in industrial saline wastewater: a literature review. Water Res 40:3671–3682. https://doi.org/10.1016/j.watres.2006.08.027
Liu B, Chen B, Zhang BY, Jing L, Zhang H, Lee K (2016) Photocatalytic degradation of polycyclic aromatic hydrocarbons in offshore produced water: effects of water matrix. J Environ Eng 142(11):04016054
Mallick S, Chakraborty J, Dutta TK (2011) Role of oxygenases in guiding diverse metabolic pathways in the bacterial degradation of low-molecular-weight polycyclic aromatic hydrocarbons: a review. Crit Rev Microbiol 37:64–90. https://doi.org/10.3109/1040841X.2010.512268
Marston CP, Pereira ZC, Ferguson J, Fischer L, Hedstrom O, Dashwood WM, Baird WM (2001) Effect of a complex environmental mixture from coal tar containing polycyclic aromatic hydrocarbons (PAH) on tumor initiation, PAH DNA binding and metabolic activation of carcinogenic PAH in mouse epidermis. Carcinogenesis 22:1077–1086. https://doi.org/10.1093/carcin/22.7.1077
Mnif S, Chamkha M, Sayadi S (2009) Isolation and characterization of Halomonas sp. Strain C2SS100, a hydrocarbon degrading bacterium under hypersaline conditions. J Appl Microbiol 107: 785–794. https://doi.org/10.1111/j.1365-2672.2009.04251.x
Mnif S, Sayadi S, Chamkha M (2014) Biodegradative potential and characterization of a novel aromatic-degrading bacterium isolated from a geothermal oil field under saline and thermophilic conditions. Int Biodeterior Biodegrad 86:258–264. https://doi.org/10.1016/j.ibiod.2013.09.015
Mukherjee S, Bardolui NK, Karim S, Patnaik VV, Nandy RK, Bag PK (2010) Isolation and characterization of a monoaromatic hydrocarbon-degrading bacterium, Pseudomonas aeruginosa from crude oil. J Environ Sci Health A Toxic Hazard Subst Environ Eng 45:1048–1053. https://doi.org/10.1080/10934529.2010.486328
Nogales B, Lanfranconi M, Pina-Villalonga JM, Bosch R (2011) Anthropogenic perturbations in marine microbial communities. FEMS Microbiol Rev 35:275–298. https://doi.org/10.1111/j.1574-6976.2010.00248.x
Piubeli F, Grossman MJ, Fantinatti-Garboggini F, Durrant LR (2012) Identification and characterization of aromatic degrading Halomonas in hypersaline produced water and COD reduction by bioremediation by the indigenous microbial population using nutrient addition. Chem Eng Trans 27:385–390. https://doi.org/10.3303/CET1227065
Pugazhendi A, Qari J, Basahi JMA, Godon JJ, Dhavamani J (2017) Role of a halothermophilic bacterial consortium for the biodegradation of PAHs and the treatment of petroleum wastewater at extreme conditions. Int Biodeterior Biodegrad 121:44–54. https://doi.org/10.1016/j.ibiod.2017.03.015
Rice EW, Baird RB, Eaton AD, Clesceri LS (eds) (2005) Standard methods for the examination of water and wastewater, 21st edn. American Public Health Association, APHA, Washington
Roy M, Khara P, Dutta TK (2012) meta-Cleavage of hydroxynaphthoic acids in the degradation of phenanthrene by Sphingobium sp. strain PNB. Microbiology 158:685–695. https://doi.org/10.4172/2155-6199.1000173
Tsai JC, Kumar M, Lin JG (2009) Anaerobic biotransformation of fluorene and phenanthrene by sulfate-reducing bacteria and identification of biotransformation pathway. J Hazard Mater 164(2–3):847–855. https://doi.org/10.1016/j.jhazmat.2008.08.101
Vasudevan N, Bharathi S, Arulazhagan P (2007) Role of plasmid in the degradation of petroleum hydrocarbon by Pseudomonas fluorescens NS1. J Environ Sci Health Part A 42(8):1141–1146. https://doi.org/10.1080/10934520701418649
Wang Y, Qian PY (2009) Conservative fragments in bacterial 16S rRNA genes and primer design for 16S ribosomal DNA amplicons in metagenomic studies. PLoS One 4:e7401. https://doi.org/10.1371/journal.pone.0007401
Zeinali M, Vossoughi M, Ardestani SK (2008) Degradation of phenanthrene and anthracene by Nocardia otitidiscaviarum strain TSH1, a moderately thermophilic bacterium. J Appl Microbiol 105(2):398–406. https://doi.org/10.1111/j.1365-2672.2008.03753.x
Acknowledgements
This project was funded by Deanship of Scientific Research (DSR) at King Abdulaziz University, Jeddah, under Grant no. G-297-150-38. The authors, therefore, acknowledge with thanks DSR for technical and financial support.
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Jamal, M.T., Pugazhendi, A. Degradation of petroleum hydrocarbons and treatment of refinery wastewater under saline condition by a halophilic bacterial consortium enriched from marine environment (Red Sea), Jeddah, Saudi Arabia. 3 Biotech 8, 276 (2018). https://doi.org/10.1007/s13205-018-1296-x
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DOI: https://doi.org/10.1007/s13205-018-1296-x