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Bacterial community of a spider, Marpiss magister (Salticidae)

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Abstract

Arthropods are associated with various microorganisms which confer benefits to their hosts. Recently, research has been conducted on bacterial communities of insects to provide an insight into the potential interactions of the symbiotic bacteria and their hosts. Spiders are interesting to study as they are perceived to be natural enemies of pests. The effect of endosymbionts on spiders has been reported, but little is known about the overall bacterial communities present in spiders. Here, we report on the characterization of bacterial communities present in the whole body of the spider Marpiss magister using Illumina sequencing of 16S rRNA amplicons. Our study shows that the most abundant phyla of bacteria included Proteobacteria, Tenericutes, Bacteroidetes and Actinobacteria. At the genus level, the most abundant genera included Rickettsia, Wolbachia, Spiroplasma, and Cardinium. Besides these dominant endosymbionts, our study also showed the existence of bacteria in the genera Arthrobacter, Novosphingobium, Acinetobacter, Pseudomonas, Aquabacterium and Sphingomonas at an abundance ranging from 0.65 to 0.84%, and the existence of bacterial in genera Lactobacillus, Sphingobium, Methylobacterium, Bradyrhizobium, Propionibacterium, Brevundimonas, Achromobacter, Microbacterium, Corynebacterium, and Flavobacterium at a slightly lower abundance ranging from 0.1 to 0.5%. Therefore, our finding indicates that endosymbionts are not the only microbiota present in the spider M. magister, and other bacterial taxa also exist in its bacterial community.

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Acknowledgements

This work was supported by the National Natural Science Fund of China (31401982, 31672317) and the Key Scientific and Technological Projects of Hubei (2016AHB003).

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Authors and Affiliations

  1. Centre for Behavioral Ecology and Evolution, College of Life Sciences, Hubei University, Wuhan, 430062, People’s Republic of China

    Lihua Zhang, Yueli Yun & Yu Peng

  2. Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan, 430062, People’s Republic of China

    Guimin Zhang & Yu Peng

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  1. Lihua Zhang
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  2. Guimin Zhang
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  3. Yueli Yun
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  4. Yu Peng
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Contributions

Yueli Yun and Guimin Zhang designed the experiments. Lihua Zhang, Yueli Yun and Yu Peng conducted the experiments and data analysis. Yueli Yun and Lihua Zhang wrote the manuscript.

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Correspondence to Yueli Yun.

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All the authors declare that they have no conflict of interest in the publication.

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Zhang, L., Zhang, G., Yun, Y. et al. Bacterial community of a spider, Marpiss magister (Salticidae). 3 Biotech 7, 371 (2017). https://doi.org/10.1007/s13205-017-0994-0

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