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Biosurfactant and enzyme mediated crude oil degradation by Pseudomonas stutzeri NA3 and Acinetobacter baumannii MN3

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Abstract

The present study focuses on the optimization of biosurfactant (BS) production using two potential biosurfactant producer Pseudomonas stutzeri NA3 and Acinetobacter baumannii MN3 and role of enzymes in the biodegradation of crude oil. The optimal conditions for P. stutzeri NA3 and A. baumannii MN3 for biodegradation were pH of 8 and 7; temperature of 30 and 40 °C, respectively. P. stutzeri NA3 and A. baumannii MN3 produced 3.81 and 4.68 g/L of BS, respectively. Gas chromatography mass spectrometry confirmed that BS was mainly composed of fatty acids. Furthermore, the role of the degradative enzymes, alkane hydroxylase, alcohol dehydrogenase and laccase on biodegradation of crude oil are explained. Maximum biodegradation efficiency (BE) was recorded for mixed consortia (86%) followed by strain P. stutzeri NA3 (84%). Both bacterial strains were found to be vigorous biodegraders of crude oil than other biosurfactant-producing bacteria due to their enzyme production capabilities and our results suggests that the bacterial isolates can be used for effective degradation of crude oil within short time periods.

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Acknowledgements

A. Rajasekar is thankful to the Department of Biotechnology ((DBT) Government of India) for the award of the Ramalingaswami re-entry Fellowship (BT/RLF/Re-entry/17/2012), Department of Science and Technology for the young scientist award (SB/YS/LS-40/2013), University Grants Commission (MRP-MAJOR-MICRO-2013-31825) and Science and Engineering Research Board, Department of Science and Technology, Government of India (EEQ/2016/000449). P. Parthipan acknowledge the DBT, Government of India for financial support through project scheme (DBT-RLF).

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Authors and Affiliations

  1. Environmental Molecular Microbiology Research Laboratory, Department of Biotechnology, Thiruvalluvar University, Serkkadu, Vellore, Tamilnadu, 632115, India

    Punniyakotti Parthipan, Punniyakotti Elumalai, Kuppusamy Sathishkumar & Aruliah Rajasekar

  2. Department of Microbial Biotechnology, Bharathiar University, Coimbatore, Tamilnadu, 641046, India

    Devaraj Sabarinathan

  3. Division of Entomology, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore, Tamilnadu, 641046, India

    Kadarkarai Murugan

  4. Thiruvalluvar University, Serkkadu, Vellore, Tamilnadu, 632115, India

    Kadarkarai Murugan

  5. Department of Agriculture, Food and Environment, University of Pisa, via del Borghetto 80, 56124, Pisa, Italy

    Giovanni Benelli

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  1. Punniyakotti Parthipan
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Parthipan, P., Elumalai, P., Sathishkumar, K. et al. Biosurfactant and enzyme mediated crude oil degradation by Pseudomonas stutzeri NA3 and Acinetobacter baumannii MN3. 3 Biotech 7, 278 (2017). https://doi.org/10.1007/s13205-017-0902-7

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