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Coumarin–gold nanoparticle bioconjugates: preparation, antioxidant, and cytotoxic effects against MCF-7 breast cancer cells

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Abstract

In recent, the conjugation of gold nanoparticles (AuNPs) with biomolecules has shown great potential especially in disease diagnostics and treatment. Taking this in account, we report the methodology involved in the conjugation of coumarin onto the surface of citrate-capped AuNPs by a simple in situ method. Herein, we systematically performed UV–Vis spectroscopy, transmission electron microscopy, dynamic light scattering, and zeta potential measurements to characterize citrate-capped AuNPs and bioconjugates. Our results demonstrate in-depth surface chemistry of bioconjugates with improved surface plasmon resonance (529 nm), morphology (near spherical shape), hydrodynamic diameter (25.3 nm) as well as surface charge (− 35 mV). Furthermore, the bioconjugates displayed dose-dependent response in scavenging free radicals and exhibited cytotoxicity against MCF-7 breast cancer cell lines. In addition, phase-contrast microscopic analysis revealed that bioconjugates promote apoptosis in cancer cells in a time-dependent manner. Overall, we ascertain the fact that this kind of bioconjugation of AuNPs with coumarin further enhances the efficacy of inorganic nanomaterials and thus make them a better bio-therapeutic candidate.

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Acknowledgements

Financial support from Early Career Research Award (ECR/2016/001456) from the Science Engineering and Research Board (SERB), New Delhi, India financially supported this work.

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Authors and Affiliations

  1. Food Chemistry and Molecular Cancer Biology Lab, Department of Animal Health and Management, Alagappa University, Science Block, Karaikudi, Tamil Nadu, 630 003, India

    Gokila Mahendran & Kumar Ponnuchamy

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  1. Gokila Mahendran
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  2. Kumar Ponnuchamy
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Correspondence to Kumar Ponnuchamy.

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Mahendran, G., Ponnuchamy, K. Coumarin–gold nanoparticle bioconjugates: preparation, antioxidant, and cytotoxic effects against MCF-7 breast cancer cells. Appl Nanosci 8, 447–453 (2018). https://doi.org/10.1007/s13204-018-0816-7

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  • DOI: https://doi.org/10.1007/s13204-018-0816-7

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