Abstract
This study was conducted to evaluate the effects of sixteen antibacterial formulations against Listeria monocytogenes in a sausage model using a standard experimental design with 4 independent factors at 2 levels (24). Four independent factors consisted of nisin (12.5–25 ppm), nitrite (100–200 ppm) and organic acid salts (1.55–3.1 %) and the mixture of Chinese cinnamon and Cinnamon bark Essential Oils (EOs) (0.025–0.05 %). Based on the analysis, utilization of low (0.025 %) or high concentration (0.05 %) of EOs in combination with low concentration of nitrite (100 ppm), organic acid salts (1.55 %), and nisin (12.5 ppm) could reduce respectively 1.5 or 2.6 log CFU/g of L. monocytogenes in sausage at day 7 of storage as compared to the control. A predictive equation was created to predict the growth of L. monocytogenes in sausage. The sensory evaluation was then performed on selected optimized formulations in cooked meat (both pork and beef sausages) with a trained jury consisting of 35 individuals, demonstrated the selected antimicrobial formulations were organoleptically acceptable. The results revealed an important role of hurdle technology to control L. monocytogenes in meat product.
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References
Abdollahzadeh E, Rezaei M, Hosseini H (2014) Antibacterial activity of plant essential oils and extracts: the role of thyme essential oil, nisin, and their combination to control Listeria monocytogene inoculated in minced fish meat. Food Control 35:177–183
Apostolidis E, Kwon YI, Shetty K (2008) Inhibition of Listeria monocytogenes by oregano, cranberry and sodium lactate combination in broth and cooked ground beef systems and likely mode of action through proline metabolism. Int J Food Microbiol 128:317–324
Blom H, Nerbrink E, Dainty R, Hagtvedt T, Borch E, Nissen H, Nesbakken T (1997) Addition of 2.5 % lactate and 0.25 % acetate controls growth of Listeria monocytogenes in vacuum-packed, sensory-acceptable servelat sausage and cooked ham stored at 4 °C. Int J Food Microbiol 38:71–76
Burt S (2004) Essential oils: their antibacterial properties and potential applications in foods—a review. Int J Food Microbiol 94:223–253
Cammack R, Joannou CL, Cui X-Y, Torres Martinez C, Maraj SR, Hughes MN (1999) Nitrite and nitrosyl compounds in food preservation. Biochimica et Biophysica Acta (BBA) - Bioenergetics 1411:475–488
Cui H, Gabriel AA, Nakano H (2010) Antimicrobial efficacies of plant extracts and sodium nitrite against Clostridium botulinum. Food Control 21:1030–1036
Dave D, Ghaly AE (2011) Meat spoilage mechanisms and preservation techniques: a critical review. Am J Agric Biol Sci 6:486
Dussault D, Vu KD, Lacroix M (2016) Development of a model describing the inhibitory effect of selected preservatives on the growth of listeria monocytogenes in a meat model system. Food Microbiol 53:115–121
Fratianni F, De Martino L, Melone A, De Feo V, Coppola R, Nazzaro F (2010) Preservation of chicken breast meat treated with thyme and balm essential oils. J Food Sci 75:M528–M535
Honikel K-O (2008) The use and control of nitrate and nitrite for the processing of meat products. Meat Sci 78:68–76
Huq T, Riedl B, Bouchard J, Salmieri S, Lacroix M (2014) Microencapsulation of nisin in alginate-cellulose nanocrystal (CNC) microbeads for prolonged efficacy against listeria monocytogenes. Cellulose 21:4309–4321
Huq T, Vu KD, Riedl B, Bouchard J, Lacroix M (2015) Synergistic effect of gamma (γ)-irradiation and microencapsulated antimicrobials against listeria monocytogenes on ready-to-eat (RTE) meat. Food Microbiol 46:507–514
Hyldgaard M, Mygind T, Meyer RL (2012) Essential oils in food preservation: mode of action, synergies, and interactions with food matrix components. Front Microbiol 3:1–24
Jayasena DD, Jo C (2013) Essential oils as potential antimicrobial agents in meat and meat products: a review. Trends Food Sci Technol 34:96–108
Manju S, Jose L, Srinivasa Gopal TK, CN R, KV L (2007) Effects of sodium acetate dip treatment and vacuum-packaging on chemical, microbiological, textural and sensory changes of Pearlspot (Etroplus suratensis) during chill storage. Food Chem 102:27–35
Millette M, Le Tien C, Smoragiewicz W, Lacroix M (2007) Inhibition of Staphylococcus aureus on beef by nisin-containing modified alginate films and beads. Food Control 18:878–884
Myers K, Montoya D, Cannon J, Dickson J, Sebranek J (2013) The effect of high hydrostatic pressure, sodium nitrite and salt concentration on the growth of Listeria monocytogenes on RTE ham and Turkey. Meat Sci 93:263–268
Neetoo H, Ye M, Chen H (2010) Bioactive alginate coatings to control listeria monocytogenes on cold-smoked salmon slices and fillets. Int J Food Microbiol 136:326–331
Nyachuba D, Donnelly C, Howard A (2007) Impact of nitrite on detection of listeria monocytogenes in selected ready-to-eat (RTE) meat and seafood products. J Food Sci 72:M267–M275
Oussalah M, Caillet S, Saucier L, Lacroix M (2007) Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria: E. coli O157:H7, salmonella typhimurium, Staphylococcus aureus and listeria monocytogenes. Food Control 18:414–420
Perumalla A, Hettiarachchy NS, Over KF, Ricke SC, Gbur EE, Zhang J, Davis B (2012) Effect of potassium lactate and sodium diacetate combination to inhibit Listeria monocytogenes in low and high fat chicken and Turkey hotdog model systems. Open Food Sci J 6:16–23
Poste LM, Mackie DA, Butler G, Larmond E (1991) Laboratory methods for sensory analysis of food. Copyright by Minister of Supply and Services Canada. ISBN 0–660–13807-7
Ramaswamy V, Cresence VM, Rejitha JS, Lekshmi MU, Dharsana KS, Prasad SP, Vijila HM (2007) Listeria–review of epidemiology and pathogenesis. J Microbiol, Immunol Infect 40:4–13
Samaxa RG, Matsheka MI, Mpoloka SW, Gashe BA (2012) Prevalence and antimicrobial susceptibility of salmonella isolated from a variety of raw meat sausages in Gaborone (Botswana) retail stores. J Food Prot 75:637–642
Savic IV (1985) Small-scale sausage production. Food and Agriculture Organization (FAO) of the United Nation
Scallan E, Hoekstra RM, Angulo FJ, Tauxe RV, Widdowson M-A, Roy SL, Jones JL, Griffin PM (2011) Foodborne illness acquired in the United States—major pathogens. Emerg Infect Dis 17:7–15
Solomakos N, Govaris A, Koidis P, Botsoglou N (2008a) The antimicrobial effect of thyme essential oil, nisin and their combination against Escherichia coli O157:H7 in minced beef during refrigerated storage. Meat Sci 80:159–166
Solomakos N, Govaris A, Koidis P, Botsoglou N (2008b) The antimicrobial effect of thyme essential oil, nisin, and their combination against listeria monocytogenes in minced beef during refrigerated storage. Food Microbiol 25:120–127
Stekelenburg FK (2003) Enhanced inhibition of Listeria monocytogenes in Frankfurter sausage by the addition of potassium lactate and sodium diacetate mixtures. Food Microbiol 20:133–137
Zacharof M, Lovitt R (2012) Bacteriocins produced by lactic acid bacteria a review article. APCBEE Procedia 2:50–56
Zhou GH, Xu XL, Liu Y (2010) Preservation technologies for fresh meat – a review. Meat Sci 86:119–128
Acknowledgments
The Québec Ministry of Agriculture, Fisheries and Food are particularly acknowledged for their financial support through the PSIA program (Programme de Soutien à l’Innovation en Agroalimentaire). Aliksir Inc. is acknowledged for providing us the Essential Oils and financial support. Finally, the authors sincerely thank Sarra Tnani for technical contributions.
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Ghabraie, M., Vu, K.D., Huq, T. et al. Antilisterial effects of antibacterial formulations containing essential oils, nisin, nitrite and organic acid salts in a sausage model. J Food Sci Technol 53, 2625–2633 (2016). https://doi.org/10.1007/s13197-016-2232-x
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DOI: https://doi.org/10.1007/s13197-016-2232-x