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Antilisterial effects of antibacterial formulations containing essential oils, nisin, nitrite and organic acid salts in a sausage model

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Abstract

This study was conducted to evaluate the effects of sixteen antibacterial formulations against Listeria monocytogenes in a sausage model using a standard experimental design with 4 independent factors at 2 levels (24). Four independent factors consisted of nisin (12.5–25 ppm), nitrite (100–200 ppm) and organic acid salts (1.55–3.1 %) and the mixture of Chinese cinnamon and Cinnamon bark Essential Oils (EOs) (0.025–0.05 %). Based on the analysis, utilization of low (0.025 %) or high concentration (0.05 %) of EOs in combination with low concentration of nitrite (100 ppm), organic acid salts (1.55 %), and nisin (12.5 ppm) could reduce respectively 1.5 or 2.6 log CFU/g of L. monocytogenes in sausage at day 7 of storage as compared to the control. A predictive equation was created to predict the growth of L. monocytogenes in sausage. The sensory evaluation was then performed on selected optimized formulations in cooked meat (both pork and beef sausages) with a trained jury consisting of 35 individuals, demonstrated the selected antimicrobial formulations were organoleptically acceptable. The results revealed an important role of hurdle technology to control L. monocytogenes in meat product.

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Acknowledgments

The Québec Ministry of Agriculture, Fisheries and Food are particularly acknowledged for their financial support through the PSIA program (Programme de Soutien à l’Innovation en Agroalimentaire). Aliksir Inc. is acknowledged for providing us the Essential Oils and financial support. Finally, the authors sincerely thank Sarra Tnani for technical contributions.

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Correspondence to Monique Lacroix.

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Ghabraie, M., Vu, K.D., Huq, T. et al. Antilisterial effects of antibacterial formulations containing essential oils, nisin, nitrite and organic acid salts in a sausage model. J Food Sci Technol 53, 2625–2633 (2016). https://doi.org/10.1007/s13197-016-2232-x

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  • DOI: https://doi.org/10.1007/s13197-016-2232-x

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