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Modulation of biogenic amines content by poly(propylene imine) dendrimers in rats

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Abstract

Biogenic amines and polyamines participate in all vital organism functions, their levels being important function determinants. Studies were performed to check whether repeated administration of poly(propylene imine) (PPI) dendrimers, synthetic macromolecules with diaminobutane core, and peripheral primary amine groups, may influence the endogenous level of amines, as represented by the two of them: spermidine, a natural derivative of diaminobutane, and histamine. The experiment was carried out on Wistar rats. Fourth generation PPI dendrimer, as well as maltotriose-modified fourth generation PPI dendrimers with (a) cationic open sugar shell and (b) neutral dense sugar shell that possess a higher biocompatibility, was used. Applying the combination of column chromatography on Cellex P and spectrofluorimetric assays of o-phthaldialdehyde, the final amine condensation products were employed to analyze tissue spermidine and histamine outside the central nervous system. Furthermore, radioenzymatic assay was used to measure histamine levels in the brain. The obtained results indicate that in some tissues, the endogenous concentrations of histamine and spermidine may be affected by dendrimers depending on their dose and type of dendrimers.

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Abbreviations

Fourth generation PPI:

Poly(propylene imine) dendrimers

BA:

Biogenic amines

PA:

Polyamines

OPT:

o-Phtalaldehyde

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Acknowledgments

Studies were funded by the project “Biological properties and biomedical applications of dendrimers” operated within the Foundation for Polish Science Team Programme cofinanced by the EU European Regional Development Fund. The work was done in the frame of the COST Action TD0802 “Dendrimers for biomedical applications.” We also thank Mr. Ireneusz Pieszynski for his help in statistical analysis.

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Authors and Affiliations

  1. Department of General Biophysics, University of Lodz, 141/143 Pomorska St., 90-236, Lodz, Poland

    Karol Ciepluch, Barbara Ziemba, Anna Janaszewska, Barbara Klajnert & Maria Bryszewska

  2. Leibniz Institute of Polymer Research Dresden, Hohe Strasse 6, D-01069, Dresden, Germany

    Dietmar Appelhans

  3. Department of Hormone Biochemistry, Medical University of Lodz, 7/9 Zeligowskiego St., 90-752, Lodz, Poland

    Wiesława Agnieszka Fogel

Authors
  1. Karol Ciepluch
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  2. Barbara Ziemba
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  3. Anna Janaszewska
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  4. Dietmar Appelhans
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  5. Barbara Klajnert
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  6. Maria Bryszewska
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  7. Wiesława Agnieszka Fogel
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Correspondence to Karol Ciepluch.

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Ciepluch, K., Ziemba, B., Janaszewska, A. et al. Modulation of biogenic amines content by poly(propylene imine) dendrimers in rats. J Physiol Biochem 68, 447–454 (2012). https://doi.org/10.1007/s13105-012-0158-y

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  • DOI: https://doi.org/10.1007/s13105-012-0158-y

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