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Experimental Basosquamous Carcinoma Model in Rats

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Abstract

Previous studies have established that 7,12-dimethylbenz(a)anthracene (DMBA) can initiate skin tumorigenesis in conventional furred mouse models by acting on hair follicle stem cells. In this work, we have developed a simple and convenient rat model of basosquamous carcinoma (BSC) using DMBA-induced carcinogenesis in male specific pathogen free (SPF) Wistar rats with no additional tumor promoter agents. The results showed that topical application of 0.1% solution of DMBA in benzene in a volume of 40 μL twice a week produced skin tumors after 8–9 months. As a result, during the 11–12th months, we obtained 15 animals with BSCs, 22 with basal cell carcinomas (BCCs), and 3 with squamous cell carcinomas (SCCs). This chemically driven skin cancer model in Wistar rats can serve as a suitable alternative to the mouse skin cancer model and can be reliably replicated as demonstrated by the experiment.

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Acknowledgments

Authors are thankful to professor D.G. Zaridze for his help in biological experiments.

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Authors and Affiliations

  1. I.M. Sechenov First Moscow State Medical University, 2-4 Bolshaya Pirogovskaya st., Moscow, 119991, Russia

    E. S. Snarskaya & E. V. Kuznetсova

  2. N.N. Blokhin Russian Cancer Research Center of the Russian Academy of Medical Sciences, Moscow, Russia, 115478

    L. N. Pylev

  3. Kazan Federal University, 18 Kremlevskaya st., Kazan, Russia, 420008

    A. A. Akhunzyanov

Authors
  1. E. S. Snarskaya
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  2. L. N. Pylev
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  3. A. A. Akhunzyanov
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  4. E. V. Kuznetсova
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Corresponding author

Correspondence to E. V. Kuznetсova.

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Snarskaya, E.S., Pylev, L.N., Akhunzyanov, A.A. et al. Experimental Basosquamous Carcinoma Model in Rats. BioNanoSci. 7, 423–427 (2017). https://doi.org/10.1007/s12668-016-0380-0

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  • DOI: https://doi.org/10.1007/s12668-016-0380-0

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