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Partial Characterization of Bacteriocins Produced by Two New Enterococcus faecium Isolated from Human Intestine

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Abstract

This study aimed at characterizing two novel bacteriocin-producing enterococcal strains isolated from human intestine. A total of 200 lactic acid bacteria were isolated from a woman stool sample. Two of them were selected for characterization due to their high antimicrobial activity against five strains of Listeria monocytogenes. The selected bacteria were identified as two different strains of Enterococcus faecium and designated MT 104 and MT 162. The bacteriocins produced by MT 104 and MT 162 were stable at different pH ranging from 2 to 11 and were active after different treatments such as heat, enzymes, detergents, and γ-irradiation. The two isolated strains exhibited some probiotic properties such as survival in simulated gastric fluid and intestinal fluid, lack of expression of bile salt hydrolase or hemolytic activity, adhesion to Caco-2 cells efficiently, and sensitivity to clinical antimicrobial agents. Thus, the two isolated strains of E. faecium could become new probiotic bacteria and their bacteriocins could be used for controlling L. monocytogenes in combination with irradiation for food preservation.

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Acknowledgments

This research was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC) and BSA Food Ingredients s.e.c/l.p (Montreal, Qc, Canada) under a research contract agreement with INRS. The authors are grateful to Nordion Inc. for irradiation treatment. M. Turgis is a scholarship recipient of the Fondation Armand-Frappier. The Institute of Nutraceuticals and Functional Foods (INAF) is acknowledged for giving a supplemented scholarship to post-doc K.D.V.

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The authors declare that they have no conflict of interest.

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  1. Research Laboratories in Sciences Applied to Food, INRS-Institut Armand-Frappier, 531, Boulevard des Prairies, Laval, QC, H7V 1B7, Canada

    Mélanie Turgis, Khanh Dang Vu & Monique Lacroix

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  1. Mélanie Turgis
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  2. Khanh Dang Vu
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  3. Monique Lacroix
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Correspondence to Monique Lacroix.

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Turgis, M., Vu, K.D. & Lacroix, M. Partial Characterization of Bacteriocins Produced by Two New Enterococcus faecium Isolated from Human Intestine. Probiotics & Antimicro. Prot. 5, 110–120 (2013). https://doi.org/10.1007/s12602-013-9129-z

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