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In vitro and in vivo study of microporous ceramics using MC3T3 cells, CAM assay and a pig animal model

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Abstract

Bone tissue engineering combines biomaterials with biologically active factors and cells to hold promise for reconstructing craniofacial defects. In this study the biological activity of biphasic hydroxyapatite ceramics (HA; a bone substitute that is a mixture of hydroxyapatite and β-tricalcium phosphate in fixed ratios) was characterized (1) in vitro by assessing the growth of MC3T3 mouse osteoblast lineage cells, (2) in ovo by using the chick chorioallantoic membrane (CAM) assay and (3) in an in vivo pig animal model. Biocompatibility, bioactivity, bone formation and biomaterial degradation were detected microscopically and by radiology and histology. HA ceramics alone demonstrated great biocompatibility on the CAM as well as bioactivity by increased proliferation and alkaline phosphatase secretion of mouse osteoblasts. The in vivo implantation of HA ceramics with bone marrow mesenchymal stem cells (MMSCs) showed de novo intramembranous bone healing of critical-size bone defects in the right lateral side of pig mandibular bodies after 3 and 9 weeks post-implantation. Compared with the HA ceramics without MMSCs, the progress of bone formation was slower with less-developed features. This article highlights the clinical use of microporous biphasic HA ceramics despite the unusually shaped elongated micropores with a high length/width aspect ratio (up to 20) and absence of preferable macropores (>100 µm) in bone regenerative medicine.

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Acknowledgments

This work was realized within the framework of the project ITMS no. 26220120066 “Centre of excellence for biomedical technologies,” which is supported by the Operational Program “Research and Development” financed through the European Regional Development Fund and within the project of the Ministry of Education VEGA no. 1/0046/16.

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Authors and Affiliations

  1. Railway Hospital in Kosice, Masarykova 1632/9, 04001, Kosice, Slovakia

    Marek Tomco

  2. Institute of Anatomy, University of Veterinary Medicine and Pharmacy in Kosice, Komenskeho 73, 04181, Kosice, Slovakia

    Eva Petrovova

  3. Institute of Materials Research, Slovak Academy of Sciences, Watsonova 47, 04001, Kosice, Slovakia

    Maria Giretova & Lubomir Medvecky

  4. Institute of Histology and Embryology, University of Veterinary Medicine and Pharmacy in Kosice, Komenskeho 73, 04181, Kosice, Slovakia

    Viera Almasiova, Katarina Holovska & Viera Cigankova

  5. Department of Stomatology and Maxillofacial Surgery, L. Pasteur University Hospital in Kosice, Rastislavova 43, 04011, Kosice, Slovakia

    Andrej Jenca Jr., Janka Jencova & Andrej Jenca

  6. Clinical Department of Horses, University of Veterinary Medicine and Pharmacy in Kosice, Komenskeho 73, 04181, Kosice, Slovakia

    Martin Boldizar

  7. Clinical Department of Swine, University of Veterinary Medicine and Pharmacy in Kosice, Komenskeho 73, 04181, Kosice, Slovakia

    Kosa Balazs

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  1. Marek Tomco
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  12. Lubomir Medvecky
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Correspondence to Eva Petrovova.

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Tomco, M., Petrovova, E., Giretova, M. et al. In vitro and in vivo study of microporous ceramics using MC3T3 cells, CAM assay and a pig animal model. Anat Sci Int 92, 569–580 (2017). https://doi.org/10.1007/s12565-016-0362-x

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  • DOI: https://doi.org/10.1007/s12565-016-0362-x

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