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Accumulation and Depuration Kinetics of Rotavirus in Mussels Experimentally Contaminated

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Abstract

Bivalve mollusks as filter-feeders concentrate in their digestive tissue microorganisms likely present in the harvesting water, thus becoming risky food especially if consumed raw or poorly cooked. To eliminate bacteria and viruses eventually accumulated, they must undergo a depuration process which efficacy on viruses is on debate. To better clarify the worth of the depuration process on virus elimination from mussels, in this study we investigated rotavirus kinetics of accumulation and depuration in Mytilus galloprovincialis experimentally contaminated. Depuration process was monitored for 9 days and virus residual presence and infectivity were evaluated by real time quantitative polymerase chain reaction, cell culture and electron microscopy at days 1, 2, 3, 5, 7, 9 of depuration. Variables like presence of ozone and of microalgae feeding were also analyzed as possible depuration enhancers. Results showed a two-phase virus removal kinetic with a high decrease in the first 24 h of depuration and 5 days necessary to completely remove rotavirus.

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Acknowledgements

We would like to thank Dr. Roberta Pellicanò for statistical analysis.

Funding

This work was supported by Grant RC015/IZSME from Italian Ministry of Health.

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Authors and Affiliations

  1. Department of Animal Health, Experimental Zooprophylactic Institute of Southern Italy, Via Salute, 2, 80055, Portici, NA, Italy

    Maria Grazia Amoroso, Denise Di Concilio, Giorgio Galiero & Giovanna Fusco

  2. Aquaculture Division, CAISIAL Center, University of Naples Federico II, Via Salute, Portici, NA, Italy

    Antonio Luca Langellotti & Anna Martello

  3. Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Via Delpino 2, Naples, Italy

    Valeria Russo

  4. Istituto Superiore Di Sanità Department of Food Safety, Nutrition and Veterinary Public Health, Viale Regina Elena 299, 00161, Rome, Italy

    Marina Monini, Ilaria Di Bartolo & Giovanni Ianiro

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  1. Maria Grazia Amoroso
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  2. Antonio Luca Langellotti
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  3. Valeria Russo
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  4. Anna Martello
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  5. Marina Monini
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Amoroso, M.G., Langellotti, A.L., Russo, V. et al. Accumulation and Depuration Kinetics of Rotavirus in Mussels Experimentally Contaminated. Food Environ Virol 12, 48–57 (2020). https://doi.org/10.1007/s12560-019-09413-0

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