Abstract
Aichi virus 1 (AiV-1) has a worldwide distribution and is associated with gastroenteritis in humans. In this study, raw sewage and mussel samples were analyzed for the presence of AiV-1 using reverse transcription-PCR (RT-PCR). Amplification and sequencing of the 3CD and VP1 genomic regions followed by phylogenetic analysis using selected genome sequences revealed the presence of AiV-1, genotype B. The results highlight the importance of further screening to evaluate the prevalence and epidemiology of this clinically important virus in South Africa.
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Acknowledgements
This work was supported by Medical Research Council (MRC, South Africa) and Research Council (RC, Rhodes University) grants. OO was supported by the Department of Public Health, Ministry of Health and wellness, Botswana. NU was supported by postgraduate fellowships from the National Research Foundation of South Africa and the German Academic Exchange Service (DAAD). The content of this publication is solely the responsibility of the authors and does not necessarily represent official views of the funders.
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Onosi, O., Upfold, N.S., Jukes, M.D. et al. The First Molecular Detection of Aichi Virus 1 in Raw Sewage and Mussels Collected in South Africa. Food Environ Virol 11, 96–100 (2019). https://doi.org/10.1007/s12560-018-9362-4
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DOI: https://doi.org/10.1007/s12560-018-9362-4