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ACC deaminase positive Enterobacter-mediated mitigation of salinity stress, and plant growth promotion of Cajanus cajan: a lab to field study

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Abstract

Salinity is a major abiotic stress that negatively impacts plant health and soil microbiota. ACC (1-aminocyclopropane carboxylic acid) deaminase producing microorganisms act as natural stress busters that protect plants from different kinds of stresses. The study focused on the isolation of potent, indigenous, multi-trait ACC deaminase producers. The shortlisted ACC deaminase producers were checked for their ability to promote growth of Cajanus cajan, and mitigate stress under laboratory conditions followed by validation of their potency in naturally saline field conditions. Physiological stress markers were assessed to evaluate the impact of salinity in plants treated with ACC deaminase producer, compared to controls. Further, the contribution of ACC deaminase in stress mitigation was demonstrated by using a chemical inhibitor for ethylene biosynthesis. This study presents a polyphasic approach, transitioning from the rhizospheric soil to the laboratory to validation in the field, and puts forth a promising eco-friendly alternative for sustainable agriculture.

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Funding

The work was supported by Science and Engineering Research Board, Department of Science and Technology, Govt. of India (Grant No. YSS/2015/001437) granted to SS. GA wishes to acknowledge his fellowship from IIT Delhi. AB wishes to acknowledge her fellowship from Science and Engineering Research Board, Department of Science and Technology, Government of India (PDF/2018/001905).

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Authors and Affiliations

  1. Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India

    Gautam Anand, Annapurna Bhattacharjee, Vijay Laxmi Shrivas, Shubham Dubey & Shilpi Sharma

  2. Centre for Rural Development and Technology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India

    Vijay Laxmi Shrivas

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  1. Gautam Anand
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  2. Annapurna Bhattacharjee
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  3. Vijay Laxmi Shrivas
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  4. Shubham Dubey
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  5. Shilpi Sharma
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Contributions

GA and SS conceptualized the study, SS acquired financial support, GA and SS designed the experiments, GA, VLS, SD and AB performed the experiments and compiled the data, SS supervised the work, GA wrote the original draft, AB and SS reviewed and edited the manuscript, all authors approved the manuscript.

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Correspondence to Shilpi Sharma.

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Anand, G., Bhattacharjee, A., Shrivas, V.L. et al. ACC deaminase positive Enterobacter-mediated mitigation of salinity stress, and plant growth promotion of Cajanus cajan: a lab to field study. Physiol Mol Biol Plants 27, 1547–1557 (2021). https://doi.org/10.1007/s12298-021-01031-0

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  • DOI: https://doi.org/10.1007/s12298-021-01031-0

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