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Screening of rice landraces for salinity tolerance at seedling stage through morphological and molecular markers

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Abstract

The present investigation was carried out to evaluate 33 rice landrace genotypes for assessment of their salt tolerance at seedling stage. Growth parameters like root length, shoot length and plant biomass were measured after 12 days of exposure to six different levels of saline solution (with electrical conductivity of 4, 6, 8, 10, 12 or 14 dS m −1). Genotypes showing significant interaction and differential response towards salinity were assessed at molecular level using 11 simple sequence repeats (SSR) markers, linked with salt tolerance quantitative trait loci. Shoot length, root length and plant biomass at seedling stage decreased with increasing salinity. However, relative salt tolerance in terms of these three parameters varied among genotypes. Out of the 11 SSR markers RM8094, RM336 and RM8046, the most competent descriptors to screen the salt tolerant genotypes with higher polymorphic information content coupled with higher marker index value, significantly distinguished the salt tolerant genotypes. Combining morphological and molecular assessment, four lanraces viz. Gheus, Ghunsi, Kuthiahara and Sholerpona were considered as true salt tolerant genotypes which may contribute in greater way in the development of salt tolerant genotypes in rice.

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Abbreviations

EC:

Electric conductivity

QTL:

Quantitative trait loci

SSR:

Simple sequence repeats

UPGMA:

Unweighted pair group method arithmetic average

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Acknowledgments

The authors are grateful to Central Research Institute for Jute and Allied Fibres (CRIJAF), Barrack pore, North 24-Parganas, West Bengal, India for supporting the molecular marker experiment and Faculty Centre for Integrated Rural Development and Management, School of Agriculture and Rural Development, Ramakrishna Mission Vivekananda University, India for providing the key research facilities.

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Authors and Affiliations

  1. Department of Agricultural Biotechnology, Faculty Centre for Integrated Rural Development and Management, School of Agriculture and Rural Development, Ramakrishna Mission Ashrama, Narendrapur, Ramakrishna Mission Vivekananda University, Kolkata, 700103, India

    Md. Nasim Ali, Lucina Yeasmin, Rupak Goswami & Somsubhra Chakraborty

  2. Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

    Saikat Gantait

  3. Sasya Shyamala Krishi Vigyan Kendra, Ramakrishna Mission Vivekananda University, Arapanch, Sonarpur, Kolkata, 700150, India

    Saikat Gantait

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  1. Md. Nasim Ali
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  2. Lucina Yeasmin
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  3. Saikat Gantait
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  4. Rupak Goswami
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  5. Somsubhra Chakraborty
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Correspondence to Md. Nasim Ali.

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Ali, M.N., Yeasmin, L., Gantait, S. et al. Screening of rice landraces for salinity tolerance at seedling stage through morphological and molecular markers. Physiol Mol Biol Plants 20, 411–423 (2014). https://doi.org/10.1007/s12298-014-0250-6

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  • DOI: https://doi.org/10.1007/s12298-014-0250-6

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