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Development of EST-based new SSR markers in seabuckthorn

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Abstract

EST-based SSR markers were developed by screening a collection of 1584 clustered ESTs of seabuckthorn (Hippophae rhamnoides). PCR primers were designed for the amplification of 30 microsatellite loci. Two to five allelic bands were displayed by nine primer pairs in H. rhamnoides genotypes and by eleven primer pairs in H. salicifolia genotypes. None of the thirty primer pairs detected polymorphism in H. tibetana genotypes. Considering the high polymorphism detected in the tested genotypes and their direct origin from the genic regions, these EST-SSR markers hold immense promise in seabuckthorn genome analysis, molecular breeding and population genetics.

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Acknowledgements

This research and AJ was supported by Defense Research and Development Organization (DRDO), India through a sponsored project (Project No. LSRB-146/FS/2008). RG thanks Indian Council of Medical Research (ICMR), India for financial support. Authors wish to thank Defense Institute of High Altitude Research (DIHAR), Leh, India for providing genotypes used in this study.

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  1. Atul Grover

    Present address: Defense Institute of Bio-Energy Research, Goraparao, Haldwani, 263139, India

Authors and Affiliations

  1. University School of Biotechnology, Guru Gobind Singh Indraprastha University, Kashmere Gate, Delhi, 110403, India

    Ankit Jain, Rajesh Ghangal, Atul Grover & Prakash C. Sharma

  2. Interdisciplinary Center for Plant Genomics, Department of Plant Molecular Biology, University of Delhi, South Campus, Benito Juarez Marg, New Delhi, 110021, India

    Saurabh Raghuvanshi

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  1. Ankit Jain
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  2. Rajesh Ghangal
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  3. Atul Grover
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  4. Saurabh Raghuvanshi
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  5. Prakash C. Sharma
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Correspondence to Prakash C. Sharma.

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Jain, A., Ghangal, R., Grover, A. et al. Development of EST-based new SSR markers in seabuckthorn. Physiol Mol Biol Plants 16, 375–378 (2010). https://doi.org/10.1007/s12298-010-0037-3

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  • DOI: https://doi.org/10.1007/s12298-010-0037-3

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