Abstract
To establish a standard of quality control and to identify reliable Achyranthis Radix, three phytoecdysones including ecdysterone (1), 25R-inokosterone (2) and 25S-inokosterone (3) were determined by quantitative HPLC/UV analysis. Three phytoecdysones were separated with an YMC J’sphere ODS C18 column (250 mm × 4.6 mm, 4 μm) by isocratic elution using 0.1% formic acid in water and acetonitrile (85:15, v/v%) as the mobile phase. The flow rate was 1.0 mL/min and the UV detector wavelength was set at 245 nm. The standards were quantified by HPLC/UV from Achyranthes bidentata Blume and Achyranthes japonica Nakai, as well as Cyathula capitata Moq. and Cyathula officinalis Kuan, which are of a different genus but are comparative herbs. The method was successfully used in the analysis of Achyranthis Radix of different geographical origin or genera with relatively simple conditions and procedures, and the assay results were satisfactory for linearity, recovery, precision, accuracy, stability and robustness. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of eighteen A. bidentata Blume samples and ten A. japonica Nakai samples. The results indicate that the established HPLC/UV method is suitable for quantitation and pattern recognition analyses for quality evaluation of Achyranthis Radix.
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Bai, Y., Yu, Z. X., Sun, S. Q., Zhu, F. Y., Wang, D., and Chen, Z. H., Determinatio of ecdysterone in Radix Achranthis Bidentatae by near infrared diffuse reflectance spectroscopy. Chin. Tradit. Herb Drugs, 36, 1391–1394 (2005).
He, Q., Ge, Z. W., Song, Y., and Cheng, Y. Y., Quality evaluation of Cortex Moutan by high performance liquid chromatography coupled with diode array detector and electrospray ionization tandem mass spectrometry. Chem. Pharm. Bull., 54, 1271–1275 (2006).
Kim, J. H., Kim, J. M., and Kang, D. H., A study on discriminative criteria of 6 kinds of Achyranthis Radix using HPLC/DAD. Kor. J. Herbology, 23, 109–116 (2008).
Korea Food and Drug Administration, Korean Pharmacopoeia IX, Part II (K. P.). Shinil Books, Seoul, p. 1159, (2008).
Li, J., Li, H. P., Li, P., and Qi, H., Simultaneous qualitation and quantification of four phytoecdysones in Radix Achranthis Bidentatae by high-performance liquid chromatography with diode array detection. Biomed. Chormatogr., 21, 823–828 (2007a).
Li, J., Li, P., Li, H. J., Song, Y., Bi, Z. M., and Li, Y. J., Simultaneous qualification and quantification of eight triterpenoids in radix Achyranthis bidentatae by high-performance liquid chromatography with evaporative light scattering detection and mass spectrometric detection. J. Sep. Sci., 30, 843–850 (2007b).
Li, Y. J., Wei, H. L., Qi, L. W., Chen, J., Ren, M. T., and Li, P., Characterization and identification of saponins in Achyranthes bidentata by rapid-resolution liquid chromatography with electrospray ionization quadrupole time-offlight tandem mass spectrometry. Rapid Commun. Mass Spectrom., 24, 2975–2985 (2010).
Liang, Y. Z., Xie, P. S., and Chan, K. J., Quality control of herbal medicines. Chromatogr. B, 812, 53–70 (2004).
Ma, Y., Che, Z. T., Bi, K. S., Wang X., and Huang, W., Determination of ecdysterone in the root of Achyranthes bidentata Bl. by Rp-HPLC. Yao Xue Xue Bao, 35, 313–315 (2000).
Park, J. S., Seong, N. S., and Lee, Y. J., Comparative study on the anti-oxidative effects of Achyranthis japonicae Radix, Achyranthis Bidentatae Radix and Cyathulae Radix. Kor. J. Herbology, 22, 155–167 (2007).
Son, K. H., Hwang, J. H., Lee, S. H., Park, J. H., Kang, J. K., Chang, S. Y., and Lee, K. S., Isolation and quantitative determination of 20-hydroxyecdysone from Achyranthis radix. Kor. J. Pharmacogn., 30, 335–339 (1999).
Song, L. R., Hong, X., and Ding, X. L., Modern Chinese Materia Medica Lexicon. People Health Publishing House Press, Beijing, p. 399, (2001).
Tanaka, K., Kuba, Y., Ina, A., Watanabe, H., and Komatsu, K., Prediction of cyclooxygenase inhibitory activity of curcuma rhizome from chromatograms by multivariate analysis. Chem. Pharm. Bull., 56, 963–940 (2008).
The Minister of Health, Labor and welfare of Japan, The Japanese Pharmacopoeia 15th Edition (J. P.). The Minister of Health, Labor and Welfare of Japan Press, Tokyo, p. 1211, (2008).
The Pharmacopoeia Commission of the PRC (C. P.). Pharmacopoeia of People’s Republic of China. Chemical Industry Press, Beijing, p. 67, (2010).
Wang, L., Xu, X. Y., Zheng, Y. M., Fu, S. Q., and Yi, Z. H., Detaermination of total phytoecdysone from Achyranthes bidentata by spectrometry. Lishizhen Med. Mater. Med. Res., 16, 18–19 (2005).
Wang, Y. Y., Zhan, Z. J., Wang, X. W., Xu, X. Y., and Zhang, H. J., Study on the fingerprints of Achyranthes bidentata Bl. by HPLC/UV/MS. Chin. Med. Mater., 26, 787–789 (2003).
Zhang, H., Zhang, Z. Z., and Wei, Y. J., An approach to content of ecdysterone in Achyranthes bidentata BL. collected in different period. J. Chin. Med. Mater., 23, 734–735 (2000).
Zhang, Y., Wei, Y., and Su, H., Determination of ecdysterone in Achyranthes bidentata Blume by RP-HPLC with ultrasonic extraction. Chin. J. Spectroscopy Lab., 19, 668–671 (2002).
Zhu, T. T., Liang, H., Zhao, Y. Y., and Wang, B., Isotation and structure identification of C-25 epimers of inokosterone from Achyranthes bidentata Blume. Yao Xue Xue Bao, 39, 913–916 (2004).
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Zhao, B.T., Jeong, S.Y., Moon, D.C. et al. High performance liquid chromatography used for quality control of Achyranthis Radix. Arch. Pharm. Res. 35, 1449–1455 (2012). https://doi.org/10.1007/s12272-012-0815-2
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DOI: https://doi.org/10.1007/s12272-012-0815-2