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Rhamnetin-induced suppression of clonal expansion during early stage of adipogenesis

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Abstract

Adipocyte differentiation plays a pivotal role in the progression of obesity which is a major risk factor for several diseases such as diabetes, hypertension and coronary heart disease. In this study, the inhibitory effect of rhamnetin, a flavonoid compound, on adipogenesis in 3T3-L1 cells was investigated. Rhamnetin decreased the accumulation of lipid droplets, and inhibited the elevation of triglyceride content in the adipocytes (IC50 = 17.3 μM). The expressions of PPARγ, C/EBPα, and perilipin, adipocyte differentiation markers, were significantly reduced by rhamnetin. Triglyceride biosynthesis and clonal expansion of adipocytes were completely inhibited during the early stage by rhamnetin. Additionally, rhamnetin significantly decreased the expression of C/EBPβ, an early stage marker. Our results indicate that suppression of clonal expansion during the early stage of adipogenesis by rhamnetin may be associated with inhibition of the C/EBPβ, C/EBPα, and PPARγ pathways.

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Authors and Affiliations

  1. College of Pharmacy and Medical Research Center, Chungbuk National University, Cheongju, 361-763, Korea

    So-Young Ji, Kyeong-Mi Choi, Youn-Sun Lee, Dong-Mi Shin, Seunghyun Lee, Kwang-Sik Yoo, Yong-Moon Lee, Yeo-Pyo Yun & Hwan-Soo Yoo

  2. Laboratory of Chemical Genomics, Korea Research Institute of Chemical Technology, Daejeon, 305-600, Korea

    Ji-Yeon Yu

  3. College of Pharmacy, Chungbuk National University, Cheongju, 361-763, Korea

    Hwan-Soo Yoo

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  1. So-Young Ji
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  2. Kyeong-Mi Choi
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  3. Youn-Sun Lee
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Correspondence to Hwan-Soo Yoo.

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These authors contributed equally to this work.

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Ji, SY., Choi, KM., Lee, YS. et al. Rhamnetin-induced suppression of clonal expansion during early stage of adipogenesis. Arch. Pharm. Res. 35, 1083–1089 (2012). https://doi.org/10.1007/s12272-012-0616-7

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  • DOI: https://doi.org/10.1007/s12272-012-0616-7

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