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R5 Peptide-based Biosilicification Using Methyltrimethoxysilane

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Abstract

We examined the performance of methyltrimethoxysilane (MTMS), a precursor of silicic acid, in the process of biosilicification induced by the R5 peptide from Cylindrotheca fusiformis. Recombinant GFP-R5 fusion protein was produced by Escherichia coli cultured at 25°C as a soluble and functional formation, but not at 37°C. MTMS-based biosilica deposits had a larger average diameter compared to tetraethyl orthosilicate (TEOS)-based deposits. Reducing phosphate concentration in the buffer system led to a decrease in the size of MTMS-based biosilica. These results provide insight into the surface modification of biosilica, and control of biosilica particle size, when using hydrophobic precursors such as MTMS.

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Author notes

  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, 790-784, Korea

    Jeong Chan Park

  2. School of Chemical Engineering, Yeungnam University, Gyeongsan, 38541, Korea

    Do Hyeon Kim & Jeong Hyun Seo

  3. School of Chemistry and Biochemistry, Yeungnam University, Gyeongsan, 38541, Korea

    Chang Sup Kim

Authors
  1. Jeong Chan Park
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  2. Do Hyeon Kim
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  3. Chang Sup Kim
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  4. Jeong Hyun Seo
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Correspondence to Jeong Hyun Seo.

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Park, J.C., Kim, D.H., Kim, C.S. et al. R5 Peptide-based Biosilicification Using Methyltrimethoxysilane. Biotechnol Bioproc E 23, 11–15 (2018). https://doi.org/10.1007/s12257-017-0451-2

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  • DOI: https://doi.org/10.1007/s12257-017-0451-2

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