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Development of ERE/DRE-dual CALUX bioassays system for monitoring estrogen- and dioxin-like persistent organic pollutants

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Abstract

Estrogen- and dioxin-like responsive element mediated Chemically Activated LUciferine gene eXpression (ERE/DRE-dual CALUX) was utilized to develop dualbioassays for simultaneously determining estrogen and dioxin levels. The devised ERE/DRE-dual CALUX bioassay system, was applied to the analysis of 103 estrogen and dioxin-related isomers, PAHs, PCBs, and pesticides. The nonylphenol highly induced estrogen-like activity (9.95 times) while benzo(k)fluoranthene, chrysene (zonerefined), and 3,3′,4,4′,5-pentachlorobiphenyl, highly induced dioxin-like activity (32.23, 42.39, and 34.47 times) respectively as compared with the untreated control. To confirm the validity of the devised ERE/DRE-dual CALUX bioassay system, we compared this bioassay with high-resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS) to assess five air samples taken in Korea. The correlation coefficient between the ERE/DRE-dual CALUX bioassay system and HRGC/HRMS method showed a good relationship (r2 = 0.92). This study indicates that ERE/DRE-dual CALUX is a simple, high-throughput, valid, and inexpensive bioassay method for screening large numbers of environmental samples.

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Authors and Affiliations

  1. Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, 790-784, Korea

    Mi-Sun Kwon, Sun-Hee Kim, Seyoung Lim, Sung Ho Ryu & Pann-Ghill Suh

  2. School of Environmental Science and Engineering, Pohang University of Science and Technology, Pohang, 790-784, Korea

    Hyo-Jin Lee, Jong-Moon Park, Sung Ho Ryu & Pann-Ghill Suh

  3. Department of Pharmacology School of Medicine, Catholic University of Daegu, Daegu, 705-718, Korea

    Jae-Ho Yang

Authors
  1. Mi-Sun Kwon
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  2. Hyo-Jin Lee
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  3. Sun-Hee Kim
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  4. Seyoung Lim
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  5. Jong-Moon Park
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  6. Sung Ho Ryu
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  7. Jae-Ho Yang
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  8. Pann-Ghill Suh
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Correspondence to Pann-Ghill Suh.

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Kwon, MS., Lee, HJ., Kim, SH. et al. Development of ERE/DRE-dual CALUX bioassays system for monitoring estrogen- and dioxin-like persistent organic pollutants. Biotechnol Bioproc E 17, 634–642 (2012). https://doi.org/10.1007/s12257-010-0392-5

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  • DOI: https://doi.org/10.1007/s12257-010-0392-5

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