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Genetic and Pathogenic Diversity of Ralstonia solanacearum Causing Potato Brown Rot in China

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Abstract

Causing potato brown rot, Ralstonia solanacearum (R. solanacearum) strains are reported as one of the most destructive bacteria to potato (Solanum tuberosum L.) in China. In this study, 113 strains were isolated from potato, collected in the four major agroecological zones in China. The study showed that 102 strains belonged to the phylotype IIB sequevar 1 (race 3 biovar 2). The 11 remaining strains belonged to the phylotype I, sequevar 13, 17, 18, 16 or 14 M, a new sequevar closely related to sequevar 14. Thirty-four strains were further characterized according to their virulence at low temperature on three wild potato species. IIB-1 strains all belonged to high and moderate virulence, while others belonged to the low virulence group, which had limited pathogenicity.

Resumen

Causante de la pudrición café, se reporta a las variantes de Ralstonia solanacearum (R. solanacearum) como una de las bacterias más destructivas en papa (Solanum tuberosum L.) en China. En este estudio, se aislaron 113 cepas en papa, que se colectaron en las cuatro zonas agroecológicas principales en China. El estudio mostró que 102 variantes pertenecían al filotipo IIB sequevar 1 (raza 3, biovar 2). Las 11 variantes restantes pertenecían al filotipo I, sequevar 13, 17, 18, 16 o 14 M, un nuevo sequevar estrechamente relacionado con el sequevar 14. Posteriormente se caracterizaron 34 cepas de acuerdo con su virulencia a baja temperatura en tres especies silvestres de papa. Todas las cepas IIB-1 pertenecieron a los grupos de virulencia alta y moderada, mientras que otras pertenecieron al grupo de baja virulencia, el cual tuvo patogenicidad limitada.

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Acknowledgements

This Project was supported by the Fundamental Research Funds for the Central Universities (Grant No. 2013PY080), the Initial Project Fund from Huazhong Agricultural University (Grant No. 12012) and the Promoting Scientific Research Cooperation and High Level Personnel Training Project in American and Canadian Areas (Grant No. 2012-1738). We thank all of those who supplied the wilted potato plants and tubers for the isolation of R. solanacearum strains and who kindly offered us the reference strains. We also thank all of the faculty members of the Potato Research Team in Huazhong Agricultural University for their support.

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Authors and Affiliations

  1. Key Laboratory of Horticultural Plant Biology, Ministry of Education /Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture /College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China

    Li Wang, Bingsen Wang, Guozhen Zhao, Xingkui Cai & Huilan Chen

  2. Plant Science Department, Macdonald Campus, McGill University, 21111 Lakeshore Road, Sainte-Anne-de-Bellevue, Quebec, H9X 3V9, Canada

    Suha Jabaji & Philippe Seguin

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  1. Li Wang
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Correspondence to Huilan Chen.

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Wang, L., Wang, B., Zhao, G. et al. Genetic and Pathogenic Diversity of Ralstonia solanacearum Causing Potato Brown Rot in China. Am. J. Potato Res. 94, 403–416 (2017). https://doi.org/10.1007/s12230-017-9576-2

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