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Crustacean hyperglycemic hormone of Portunus trituberculatus: evidence of alternative splicing and potential roles in osmoregulation

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Cell Stress and Chaperones Aims and scope

Abstract

The crustacean hyperglycemic hormone (CHH) gene of Portunus trituberculatus (Pt-CHH) consists of four exons and three introns spanning 3849 bp in size and generating two mature mRNA, Pt-CHH1, and Pt-CHH2. The primary gene transcript produces a cDNA encoding for the putative Pt-CHH2 from exons 1, 2, 3, and 4 and an alternative transcript encodes for a putative Pt-CHH1 peptide from exons 1, 2, and 4. A promoter fragment of about 3 kb was obtained by genomic walking. The tissue-specific expression pattern is examined by reverse transcriptase chain reaction, and the results show that Pt-CHH1 is detected in the eyestalk, brain, muscle, and blood. However, Pt-CHH2 is detected in the ganglia thoracalis and gill. The results indicate that the expression of Pt-CHH2 in the gill might suggest a potential role in osmoregulation. The Pt-CHH transcript level in the gill increases when the crab is exposed to low salinity. The injection of dsRNA for Pt-CHH causes a significant reduction in Pt-CHH2 transcript level and the activity of Na+/K+-ATPase, and carbonic anhydrase (CA) show a serious decrease. In conclusion, this study provides molecular evidence to support the osmoregulatory function of Pt-CHH2.

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Funding

This research was supported by the National Natural Science Foundation of China (grant Nos 41776160 and 41576147), Efficient Eco Agriculture Innovation Project of Taishan Leading Talent Project (No. LJNY2015002), Shandong Province Key Development Program for Research (2018GSF121030), Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology (2016LMFS-A12), Qingdao Applied Basic Research Project (17-1-1-95-jch), Special Scientific Research Funds for Central Non-profit Institutes, Yellow Sea Fisheries Research Institute (Project 20603022018027), and the Key Research and Development Plan of Jiangsu Province (BE2017325).

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Authors and Affiliations

  1. Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, P.R.China, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China

    Dongfang Sun, Jianjian Lv, Baoquan Gao, Ping Liu & Jian Li

  2. Laboratory for Marine Fisheries and Aquaculture, Qingdao National Laboratory for Marine Science and Technology, No. 1 Wenhai Road, Aoshanwei Town, Jimo, Qingdao, China

    Jianjian Lv, Baoquan Gao, Ping Liu & Jian Li

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  1. Dongfang Sun
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  2. Jianjian Lv
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  3. Baoquan Gao
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  4. Ping Liu
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  5. Jian Li
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Correspondence to Ping Liu.

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Sun, D., Lv, J., Gao, B. et al. Crustacean hyperglycemic hormone of Portunus trituberculatus: evidence of alternative splicing and potential roles in osmoregulation. Cell Stress and Chaperones 24, 517–525 (2019). https://doi.org/10.1007/s12192-019-00980-6

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  • DOI: https://doi.org/10.1007/s12192-019-00980-6

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