Abstract
Cysteine (Cys) can rapidly and sensitively react with 12 polycytosine-templated silver nanoclusters (polyC12-AgNCs) via the formation of Ag–S bond and enhance the fluorescence of polyC12-AgNCs. Herein, a smart and enzyme-free biosensing platform for ultrasensitive detection of Salmonella Typhimurium (S. Typhimurium) has been constructed by the ingenious coupling of Y-shaped G-quadruplex DNAzyme (GQH DNAzyme) with polyC12-AgNCs. It is worth mentioning that the self-assembly Y-shaped GQH DNAzyme has been utilized for the oxidation of Cys to control the fluorescence signal of polyC12-AgNCs. As expected, satisfactory analytical results toward S. Typhimurium were achieved with a good linear relationship in the range of 10 cfu·mL−1 to 5.0 × 105 cfu·mL−1 and an outstanding detection limit of 6.3 cfu·mL−1. Emphatically, by replacing the corresponding aptamer for target recognition, this strategy can further extend for detection of various analytes. Thus, the fluorescence sensing strategy based on entropy-driven and self-assembly Y-shaped GQH DNAzyme indeed creates a versatile platform for S. Typhimurium analysis.
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The data supporting the results of this manuscript are available from the authors upon reasonable request.
References
Chen J, Wen J, Yang G, Zhou S (2015) A target-induced three-way G-quadruplex junction for 17beta-estradiol monitoring with a naked-eye readout. Chem Commun (camb) 51(62):12373–12376. https://doi.org/10.1039/c5cc04347f
Chen X, Yang L, Liang S, Dang P, Jin D, Cheng Z, Lin J (2021) Entropy-driven strand displacement reaction for ultrasensitive detection of circulating tumor DNA based on upconversion and Fe3O4 nanocrystals. Sci China Mater 64(10):2593–2600. https://doi.org/10.1007/s40843-020-1649-1
Dang W, Luo R, Fan J, Long Y, Tong C, Xiao F, Xiong X, Liu B (2020) RNase A activity analysis and imaging using label-free DNA-templated silver nanoclusters. Talanta 209:120512. https://doi.org/10.1016/j.talanta.2019.120512
Duan YF, Ning Y, Song Y, Deng L (2014) Fluorescent aptasensor for the determination of Salmonella typhimurium based on a graphene oxide platform. Microchim Acta 181(5–6):647–653. https://doi.org/10.1007/s00604-014-1170-4
Gu C, Kong X, Liu X, Gai P, Li F (2019) Enzymatic biofuel-cell-based self-powered biosensor integrated with DNA amplification strategy for ultrasensitive detection of single-nucleotide polymorphism. Anal Chem 91(13):8697–8704. https://doi.org/10.1021/acs.analchem.9b02510
Guo Y, Chen J, Cheng M, Monchaud D, Zhou J, Ju H (2017) Thermophilic tetramolecular G-quadruplex/hemin DNAzyme. Angew Chem Int Ed Engl 56(52):16636–16640. https://doi.org/10.1002/anie.201708964
Ilhan H, Tayyarcan EK, Caglayan MG, Boyaci IH, Saglam N, Tamer U (2021) Replacement of antibodies with bacteriophages in lateral flow assay of Salmonella Enteritidis. Biosens Bioelectron 189:113383. https://doi.org/10.1016/j.bios.2021.113383
Ji R, Niu W, Chen S, Xu W, Ji X, Yuan L, Zhao H, Geng M, Qiu J, Li C (2019) Target-inspired Pb(2+)-dependent DNAzyme for ultrasensitive electrochemical sensor based on MoS2-AuPt nanocomposites and hemin/G-quadruplex DNAzyme as signal amplifier. Biosens Bioelectron 144:111560. https://doi.org/10.1016/j.bios.2019.111560
Leng X, Wang Y, Li R, Liu S, Yao J, Pei Q, Cui X, Tu Y, Tang D, Huang J (2018) Circular exponential amplification of photoinduced electron transfer using hairpin probes, G-quadruplex DNAzyme and silver nanocluster-labeled DNA for ultrasensitive fluorometric determination of pathogenic bacteria. Microchim Acta 185(3):168. https://doi.org/10.1007/s00604-018-2698-5
Li Z, Yang H, Sun L, Qi H, Gao Q, Zhang C (2015) Electrogenerated chemiluminescence biosensors for the detection of pathogenic bacteria using antimicrobial peptides as capture/signal probes. Sensors Actuators b: Chem 210:468–474. https://doi.org/10.1016/j.snb.2015.01.011
Li C, Wei CJS, Chemical AB (2017a) DNA-Templated Silver Nanocluster as a Label-Free Fluorescent Probe for the Highly Sensitive and Selective Detection of Mercury Ions 242:563–568
Li X, Zhang H, Tang Y, Wu P, Xu S, Zhang X (2017b) A both-end blocked peroxidase-mimicking DNAzyme for low-background chemiluminescent sensing of miRNA. ACS Sens 2(6):810–816. https://doi.org/10.1021/acssensors.7b00178
Li H, Chang J, Gai P, Li F (2018) Label-free and ultrasensitive biomolecule detection based on aggregation induced emission fluorogen via target-triggered hemin/G-quadruplex-catalyzed oxidation reaction. ACS Appl Mater Interfaces 10(5):4561–4568. https://doi.org/10.1021/acsami.7b18676
Li J, Yuan T, Yang T, Xu L, Zhang L, Huang L, Cheng W, Ding S (2018) DNA-grafted hemin with preferable catalytic properties than G-quadruplex/hemin for fluorescent miRNA biosensing. Sensors Actuators b: Chem 271:239–246. https://doi.org/10.1016/j.snb.2018.05.045
Li M Luan Z Liu Y Yang C Wang Y Ma C Shi C (2021) Ultrafast bacterial cell lysis using a handheld corona treater and loop-mediated isothermal amplification for rapid detection of foodborne pathogens. Food Control 128.https://doi.org/10.1016/j.foodcont.2021.108178
Lu CH, Qi XJ, Orbach R, Yang HH, Mironi-Harpaz I, Seliktar D, Willner I (2013) Switchable catalytic acrylamide hydrogels cross-linked by hemin/G-quadruplexes. Nano Lett 13(3):1298–1302. https://doi.org/10.1021/nl400078g
Ou S, Xu T, Liu X, Yu X, Li R, Deng J, Yuan J, Chen Y (2018) Rapid and ultrasensitive detection of microRNA based on strand displacement amplification-mediated entropy-driven circuit reaction. Sensors Actuators b: Chem 255:3057–3063. https://doi.org/10.1016/j.snb.2017.09.130
Pang B, Fu K, Liu Y, Ding X, Hu J, Wu W, Xu K, Song X, Wang J, Mu Y, Zhao C, Li J (2018) Development of a self-priming PDMS/paper hybrid microfluidic chip using mixed-dye-loaded loop-mediated isothermal amplification assay for multiplex foodborne pathogens detection. Anal Chim Acta 1040:81–89. https://doi.org/10.1016/j.aca.2018.07.024
Puskal Kunwar JH, Bautista G, Ras RHA, Toivonen J (2014) Direct laser writing of photostable fluorescent silver nanoclusters in polymer films. ACS Nano 8:11165–11171
Roth-Konforti M, Green O, Hupfeld M, Fieseler L, Heinrich N, Ihssen J, Vorberg R, Wick L, Spitz U, Shabat D (2019) Ultrasensitive detection of Salmonella and Listeria monocytogenes by small-molecule chemiluminescence probes. Angew Chem Int Ed Engl 58(30):10361–10367. https://doi.org/10.1002/anie.201904719
Shi H, Cui J, Sulemana H, Wang W, Gao L (2021) Protein detection based on rolling circle amplification sensors. Luminescence 36(4):842–848. https://doi.org/10.1002/bio.4017
Sun H, Yao F, Su Z, Kang XF (2020) Hybridization chain reaction (HCR) for amplifying nanopore signals. Biosens Bioelectron 150:111906. https://doi.org/10.1016/j.bios.2019.111906
Sun W, Wang Y, Zhu Z, Wang Y, Zhang M, Jiang L, Liu S, Yu J, Huang J (2021) Accurate and nonpurified identification of extracellular vesicles using dual-binding recognition mode. Anal Chem 93(36):12383–12390. https://doi.org/10.1021/acs.analchem.1c02259
Wang X, Zhu P, Pi F, Jiang H, Shao J, Zhang Y, Sun X (2016) A Sensitive and simple macrophage-based electrochemical biosensor for evaluating lipopolysaccharide cytotoxicity of pathogenic bacteria. Biosens Bioelectron 81:349–357. https://doi.org/10.1016/j.bios.2016.03.007
Wang Z, Zhao J, Bao J, Dai Z (2016) Construction of metal-ion-free G-quadruplex-hemin DNAzyme and its application in S1 nuclease detection. ACS Appl Mater Interfaces 8(1):827–833. https://doi.org/10.1021/acsami.5b10165
Yao Y, Li N, Zhang X, Ong’achwa Machuki J, Yang D, Yu Y, Li J, Tang D, Tian J, Gao F (2019) DNA-templated silver nanocluster/porphyrin/MnO2 platform for label-free intracellular Zn(2+) imaging and fluorescence-/magnetic resonance imaging-guided photodynamic therapy. ACS Appl Mater Interfaces 11(15):13991–14003. https://doi.org/10.1021/acsami.9b01530
Yousefi H, Ali MM, Su HM, Filipe CDM, Didar TF (2018) Sentinel wraps: real-time monitoring of food contamination by printing DNAzyme probes on food packaging. ACS Nano 12(4):3287–3294. https://doi.org/10.1021/acsnano.7b08010
Zhang Y, Cai Y, Qi Z, Lu L, Qian Y (2013) DNA-templated silver nanoclusters for fluorescence turn-on assay of acetylcholinesterase activity. Anal Chem 85(17):8455–8461. https://doi.org/10.1021/ac401966d
Zhang H, Ma X, Liu Y, Duan N, Wu S, Wang Z, Xu B (2015) Gold nanoparticles enhanced SERS aptasensor for the simultaneous detection of Salmonella typhimurium and Staphylococcus aureus. Biosens Bioelectron 74:872–877. https://doi.org/10.1016/j.bios.2015.07.033
Zhang Y, Mu F, Duan Y, Li Q, Pan Y, Du H, He P, Shen X, Luo Z, Zhu C, Wang L (2020) Label-free analysis of H5N1 virus based on three-segment branched DNA-templated fluorescent silver nanoclusters. ACS Appl Mater Interfaces 12(43):48357–48362. https://doi.org/10.1021/acsami.0c14509
Zhu H, Zhao G, Wang SQ, Dou W (2017) Photometric sandwich immunoassay for Salmonella pullorum and Salmonella gallinarum using horseradish peroxidase and magnetic silica nanoparticles. Microchim Acta 184(6):1873–1880. https://doi.org/10.1007/s00604-017-2241-0
Funding
This work was supported by the National Natural Science Foundation of China (32072330), the Central Government Guides Local Science and Technology Development Projects of Shandong Province (YDZX20203700001955), the Jinan City “20 Items in Colleges” Funds (2019GXRC056), and the Program for Taishan Scholer of Shandong Province (ts201712048).
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J. L. and S. L. contributed equally to this work. Jingjing Li and Shasha Li: methodology; data curation; writing—original draft. Xu Wang: project administration. Zhixue Zhu: software, formal analysis. Qingxin Zhang: data curation. Su Liu: writing—review and editing; methodology; funding acquisition. Yu Wang: writing—review and editing; methodology; funding acquisition. Jiadong Huang: writing—review and editing; methodology; funding acquisition.
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Jingjing Li declares that she has no conflict of interest. Shasha Li declares that he has no conflict of interest. Xu Wang declares that he has no conflict of interest. Zhixue Zhu declares that he has no conflict of interest. Qingxin Zhang declares that she has no conflict of interest. Su Liu declares that she has no conflict of interest. Yu Wang declares that she has no conflict of interest. Jiadong Huang declares that he has no conflict of interest.
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Li, J., Li, S., Wang, X. et al. Responsive Cysteine-Lighted Silver Nanoclusters Regulated by Highly Catalytic G-Quadruplex DNAzyme for Ultrasensitive Detection of Salmonella Typhimurium. Food Anal. Methods 15, 2633–2641 (2022). https://doi.org/10.1007/s12161-022-02300-8
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DOI: https://doi.org/10.1007/s12161-022-02300-8